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Millipore Anti-Tip60 Antibody, Clone 3f9 Is A Highly Specific Mouse Monoclonal Antibody, That Targets Tip60 & Has Been Tested In Western Blotting & Ihc. - Mill (Additional S&H or Hazmat Fees May Apply)
TIP60 protein or also known as Histone acetyltransferase KAT5, 60kDa Tat-interactive protein, Histone acetyltransferase HTATIP, HIV-1 Tat interactive protein, Lysine acetyltransferase 5, or cPLA(2)-interacting protein and encoded by the KAT5/HTATIP/TIP60 gene is a histone acetyl transferase that forms the catalytic subunit of the NuA4 histone acetyltransferase complex which is involved in transcriptional activation of a number of genes via the acetylation of histone H4 and H2A. TIP60 plays a role in DNA repair, growth arrest and senescence, apoptosis, chromatin remodeling, and tumor suppression. First discovered as protein that interacted with HIV-1 Tat resulting in TIP60 becoming polyubiquitinated and degraded (which prevented apoptosis of the infected cell), TIP60 is now known to interact with a number of other intracellular proteins including HDAC7A, CREB1, BCL3, Androgen receptor and PLA2G4A. TIP60 is largely localized to the nucleus or nuclear envelope in most tissues. TIP60 is also necessary for oncogene and proto-oncogene mediated growth induction seen in many cancers. EMD-Millipore’s Anti-TIP60 mouse monoclonal antibody has been tested in western blot on recombinant TIP60 protein and in paraffin embedded immunohistochemistry on human liver carcinoma, rectum carcinoma, normal medulla and normal interbrain tissue sections.
Product Information
Format
Ascites
Control
Human recombinant TIP60 protein
Presentation
Mouse monoclonal IgG1 ascitic fluid containing up to 0.1% sodium azide.
Quality Level
MQ100
Applications
Application
Anti-TIP60 Antibody, clone 3F9 is a highly specific mouse monoclonal antibody, that targets Tip60 & has been tested in western blotting & IHC.
Key Applications
Western Blotting
Immunohistochemistry
Application Notes
Immunohistochemistry Analysis: A 1:200-1,000 dilution from a representative lot detected TIP60 in liver carcinoma, rectum carcinoma, normal medulla and interbrain tissues.
Optimal working dilutions must be determined by end user.
Biological Information
Immunogen
Purified recombinant fragment of human TIP60 expressed in E. Coli.
Clone
3F9
Host
Mouse
Isotype
IgG2b
Species Reactivity
Human
Antibody Type
Monoclonal Antibody
Gene Symbol
TIP60
KAT5
HTATIP
Purification Method
Unpurified
UniProt Number
P63279
Molecular Weight
~60 kDa observed. Uncharacterized bands may appear in some lysate(s).
Product Usage Statements
Quality Assurance
Evaluated by Western Blotting in human recombinant TIP60 protein.
Western Blotting Analysis: A 1:500-2,000 dilution of this antibody detected human recombinant TIP60 protein.
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions
Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
