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Millipore Detect P31comet Using This Mouse Monoclonal Antibody, Anti-P31comet Antibody, Clone E29.19.14 Validated For Use In Western Blotting & Ip. - Mill (Additional S&H or Hazmat Fees May Apply)
p31comet is also called MAD2L1-binding protein (MAD2L1BP) and Caught by MAD2 protein (CMT2). p31comet silences the spindle checkpoint and allows mitosis to proceed through anaphase by binding MAD2L1 after it has become dissociated from the MAD2L1-CDC20 complex. p31comet is expressed throughout the cell cycle, with levels increasing and then remaining constant until late mitosis after which they drop.
Product Information
Format
Purified
Presentation
Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality Level
MQ100
Applications
Application
Detect p31comet using this mouse monoclonal antibody, Anti-p31comet Antibody, clone E29.19.14 validated for use in western blotting & IP.
Key Applications
Western Blotting
Immunoprecipitation
Application Notes
Western Blotting Analysis: A representative lot from an independent laboratory detected p31comet in HeLa cells arrested in prometaphase (Mansfeld, J., et al. (2011). Nat Cell Biol. 13(10):1234-1243.).
Immunoprecipitation Analysis: A representative lot from an independent laboratory detected p31comet in HeLa cell lysates, which were untreated (asynchronous), arrested in nocodazole for 16 hr, or released in fresh medium for 2 hr (Varetti, G., et al. (2011). 44(5):710-720.).
Biological Information
Immunogen
Recombinant protein corresponding to human p31comet.
Clone
E29.19.14
Concentration
Please refer to the Certificate of Analysis for the lot-specific concentration.
Host
Mouse
Isotype
IgG1κ
Species Reactivity
Human
Antibody Type
Monoclonal Antibody
Entrez Gene Number
NP_001003690
Gene Symbol
MAD2L1BP
CMT2
Purification Method
Protein G Purified
UniProt Number
Q15013
Molecular Weight
35 kDa observed
Product Usage Statements
Quality Assurance
Evaluated by Western Blotting in HeLa cell lysate.
Western Blotting Analysis: 0.5 µg/mL of this antibody detected p31comet in 10 µg of HeLa cell lysate.
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
