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Millipore Anti-Mitf Antibody, Clone 3d1.2 Is An Antibody Against Mitf For Use In Western Blotting & Ihc. - Mill (Additional S&H or Hazmat Fees May Apply)
Microphthalmia-associated transcription factor (MiTF) is a basic helix loop helix leucine zipper (b HLH ZIP) transcription factor implicated in pigmentation, and development of mast cells and bone. Mutations in MiTF cause auditory pigmentary syndromes in humans, such as Waardenburg syndrome type II and type IIa, and Tietz syndrome. There are two known isoforms of MiTF, differing by 66 amino acids at the N-terminus. Shorter forms are expressed in melanocytes and run as two bands at 52 kDa and 56 kDa, whereas the longer Mi form runs as a cluster of bands at 60-70 kDa in osteoclasts and in B16 melonoma cells (but not other melanoma cell lines), as well as mast cells and heart. MiTF plays a critical role in the differentiation of various cell types as neural crest-derived melanocytes, mast cells, osteoclasts and optic cup-derived retinal pigment epithelium.
Anti-MiTF Antibody, clone 3D1.2 is an antibody against MiTF for use in Western Blotting & IHC.
Key Applications
Western Blotting
Immunohistochemistry
Application Notes
Immunohistochemistry Analysis: A 1:100 dilution of this antibody detected MiTF in human melanoma tissue.
Biological Information
Immunogen
GST-tagged recombinant protein corresponding to human MiTF.
Clone
3D1.2
Host
Mouse
Isotype
IgMκ
Species Reactivity
Human
Mouse
Antibody Type
Monoclonal Antibody
Entrez Gene Number
NP_000239
Gene Symbol
MITF
BHLHE32
UniProt Number
O75030
Molecular Weight
~52 kDa observed. Multiple isoforms between 46-56 kDa are known to exist.
Product Usage Statements
Quality Assurance
Evaluated by Western Blot in mouse brain tissue lysate.
Western Blot Analysis: A 1:500 dilution of this antibody detected MiTF in 10 µg of mouse brain tissue lysate.
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
