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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H | WB, ICC, IP | M | Purified | Monoclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | MABE885 |
| Description | Anti-Lin-28B, clone 15G3 Antibody |
| Alternate Names |
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| Background Information | Lin28 is a developmentally regulated RNA binding protein (RBP). It’s down regulation is mediated in part by Lin4 and miR-125b, the mammalian micro-RNA homolog of Lin4. It is known to be negatively regulated by retinoic acid and act as a translational enhancer by driving specific mRNAs to polysomes and thus increasing the efficiency of protein synthesis by increasing the number of initiation events per mRNA molecule. Lin28 is necessary and sufficient for selectively blocking the cleavage processing of pri-let-7 miRNAs in embryonic cells (Viswanathan, 2008). Lin28 is a negative regulator of miRNA biogenesis and may play a role in blocking miRNA-mediated differentiation in stem cells and in certain cancers (Viswanathan, 2008). The mechanism by which miR-125b represses Lin28 in mammalian cells appears to involve reductions not only in translational efficiency, but also in mRNA abundance (Wu, 2005). Lin28 has shown to be essential for skeletal muscle differentiation through the translational upregulation of IGF2 expression. It is known to be expressed in testis, placenta, and embryonic stem (ES) cells. |
| Product Information | |
|---|---|
| Format | Purified |
| Presentation | Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Anti-Lin-28B, clone 15G3 | MABE885 is an antibody against Lin-28B for use in Western Blotting, Immunocytochemistry, Immunoprecipitation. |
| Key Applications |
|
| Application Notes | Western Blotting Analysis: 1.0 µg/mL from a representative lot detected Lin28B in 10 µg of NTera2 cell lysate. Immunocytochemistry Analysis: A representative lot detected Lin28B in K-562 cells (Prof. N. Kim, Seoul National University). Immunoprecipitation Analysis: A representative lot immunoprecipitated Lin28B in Huh7 cell lysate (Prof. N. Kim, Seoul National University). |
| Biological Information | |
|---|---|
| Immunogen | Recombinant protein corresponding to human Lin28B. |
| Clone | 15G3 |
| Concentration | Please refer to lot specific datasheet. |
| Host | Mouse |
| Specificity | This antibody does not cross react with Lin-28A. |
| Isotype | IgG1κ |
| Species Reactivity |
|
| Antibody Type | Monoclonal Antibody |
| Entrez Gene Number |
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| Gene Symbol |
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| Purification Method | Protein G Purified |
| UniProt Number |
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| Molecular Weight | ~30 kDa observed. Uniprot describes an isoform produced by alternative splicing at ~20 kDa. |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Western Blotting in HepG2 cell lysate. Western Blotting Analysis: 1.0 µg/mL of this antibody detected Lin28B in 10 µg of HepG2 cell lysate. |
| Usage Statement |
|
| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at 2-8°C from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 100 µg |