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Ikaros, also known as IKZF1, is a transcription factor that is important for the regulation of lymphopoiesis and also the development of the immune system. It has also been linked to adult and fetal erythroid differentiation in humans. Ikaros has been shown to induce local chromatin accessibility during CD4+ T cell differentiation and therefore, activating Th2 expression.
Product Information
Format
Purified
Presentation
Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality Level
MQ100
Applications
Application
Anti-Ikaros, clone Ik14 is an antibody against Ikaros for use in WB, ChIP, ChIP-seq.
Key Applications
Western Blotting
Chromatin Immunoprecipitation (ChIP)
ChIP-seq
Application Notes
Western Blotting Analysis: 0.5 µg/mL from a representative lot detected Ikaros in 10 µg of HL-60 and human lymph node lysate. Chromatin Immunoprecipitation (ChIP): A representative lot of this antibody as part of a blend Ikaros clones Ik14 and E9 (catalogue numbers MABE913 and MABE912 respectively) was demonstrated to perform in ChIP. See Zhang et al. (2012). ChIP-seq Analsysis: A representative lot of this antibody as part of a blend Ikaros clones E9 and Ik14 (catalogue number MABE912 and MABE913 respectively) was demonstrated to perform in ChIP-seq. See Zhang et al. (2012).
Biological Information
Immunogen
Recombinant protein corresponding to Human Ikaros.
Clone
Ik14
Concentration
Please refer to lot specific datasheet.
Host
Mouse
Isotype
IgG1κ
Species Reactivity
Human
Antibody Type
Monoclonal Antibody
Entrez Gene Number
NP_001207694
Gene Symbol
IKZF1
IK1
IKAROS
LYF1
ZNFN1A1
Purification Method
Protein G Purified
UniProt Number
Q13422
Molecular Weight
~60 kDa observed
Product Usage Statements
Quality Assurance
Evaluated by Western Blotting in a Jurkat cell lysate.
Western Blotting Analysis: A 0.5 µg/mL dilution of this antibody detected Ikaros in 10 µg of Jurkat cell lysate.
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
