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Millipore Anti-Sae2 Antibody, Clone 7h8.1 | Mabe927 Is An Antibody Against Sae2 Antibody For Use In Western Blotting, Immunohistochemistry. - Mill (Additional S&H or Hazmat Fees May Apply)
SUMO-activating enzyme subunit 2, also known as Anthracycline-associated resistance ARX, or Ubiquitin-like 1-activating enzyme E1B, and encoded by the gene name UBA2 or SAE2 or UBLE1B, belongs to the ubiquitin-activating E1 family. SUMOs are a family of small, related proteins (SUMO-1/2/3/4) that can be enzymatically attached to a target protein by a post-translational modification process termed sumoylation, a major regulator of protein function in cellular processes such as nuclear transport, transcriptional regulation, apoptosis and protein stability. This sumoylation is effected by the heterodimeric enzyme SAE1/SAE2 and the SUMO-1-conjugating enzyme Ubch9. The sumoylation pathway mediated by SAE1/SAE2 is distinct from other ubiquitin-like protein (Ubl) pathways.
Product Information
Format
Purified
Presentation
Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality Level
MQ100
Applications
Application
Anti-SAE2 Antibody, clone 7H8.1 | MABE927 is an antibody against SAE2 Antibody for use in Western Blotting, Immunohistochemistry.
Key Applications
Western Blotting
Immunohistochemistry
Application Notes
Immunohistochemistry Analysis: A 1:250-1,000 dilution from a representative lot detected SAE2 in human kidney and rat testis tissue.
Biological Information
Immunogen
GST-tagged recombinant protein corresponding to human SAE2.
Clone
7H8.1
Concentration
Please refer to lot specific datasheet.
Host
Mouse
Isotype
IgG1κ
Species Reactivity
Human
Mouse
Rat
Antibody Type
Monoclonal Antibody
Entrez Gene Number
NP_005490
Gene Symbol
UBA2
SAE2
UBLE1B
Purification Method
Protein G Purified
UniProt Number
Q9UBT2
Molecular Weight
~80 kDa observed. The calculated molecular weight is 72 kDa, however SAE2 has been shown as a ~70-90 kDa band in western blots (Truong, K., et al. (2012). J. Biol. Chem. 287(51):42611-42619).
Product Usage Statements
Quality Assurance
Evaluated by Western Blotting in Neuro-2a cell lysate.
Western Blotting Analysis: 1.0 µg/mL of this antibody detected SAE2 in 10 µg of Neuro-2a cell lysate.
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
