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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H | WB, DB, ICC, ELISA, ChIP, ChIP-seq | R | Purified | Monoclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | MABE941 |
| Replaces | MABE13 |
| Description | Anti-phospho Histone H3.1 (Ser28), clone 5D10D4 Antibody |
| Alternate Names |
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| Background Information | Histone H3 is one of the five main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N-terminal tail, H3 is involved with the structure of the nucleosomes of the beads on a string structure. The N-terminal tail of histone H3 protrudes from the globular nucleosome core and can undergo several different types of epigenetic modifications that influence cellular processes. These modifications include the covalent attachment of methyl or acetyl groups to lysine and arginine amino acids and the phosphorylation of serine or threonine. High levels of phosphorylation of Histone H3 are associated with mitosis. Consistent with this role, Histone H3 phosphorylated at threonine 3 has been shown to be recognized by the BIR domain Survivin, a member of the inhibitor of apoptosis (IAP) family. |
| Product Information | |
|---|---|
| Format | Purified |
| Presentation | Purified rat monoclonal IgG2aκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | This Anti-phospho Histone H3.1 (Ser28), clone 5D10D4 Antibody is validated for use in Western Blotting and Dot Blot and Immunocytochemistry and ELISA and Chromatin Immunoprecipitation (ChIP) and ChIP-seq for the detection of phospho Histone H3.1 (Ser28). |
| Key Applications |
|
| Application Notes | Chromatin Immunoprecipitation (ChIP): A representative lot of this antibody was reported to be suitable for ChIP. Personal communication Dr. T. Tachibana, Cell Engineering Corp. Osaka City, Japan. ChIP-seq: representative lot of this antibody was reported to be suitable for ChIP-seq. Personal communication Dr. T. Tachibana, Cell Engineering Corp. Osaka City, Japan. Dot Blot Specificity Analysis: A representative lot of this antibody at a concentration of 0.5ug/mL was evaluated for cross reactivity with a panel of modified and unmodified peptides. Immunocytochemistry: A representative lot of this antibody was reported to detect Histone H3.1 phosphorylated at serine 28 in Mitotic DM4 cells. See Yoshimi et al. (2013) ELISA: A representative lot of this antibody was used in ELISA against a panel of phophorylated and unmodified peptides to demonstrate specificity. See Yoshimi et al. (2013). |
| Biological Information | |
|---|---|
| Immunogen | KLH-conjugated linear peptide corresponding to human phospho Histone H3.1 (Ser28). |
| Clone | 5D10D4 |
| Concentration | Please refer to lot specific datasheet. |
| Host | Rat |
| Isotype | IgG2aκ |
| Species Reactivity |
|
| Antibody Type | Monoclonal Antibody |
| Entrez Gene Number |
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| Gene Symbol |
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| Purification Method | Protein G Purified |
| UniProt Number |
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| Molecular Weight | ~ 17 kDa observed |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Western Blotting in a nocodozole treated HeLa cell lysate. Western Blotting Analysis: A 1 µg/mL dilution of this antibody detected phospho Histone H3.1 (Ser28) in a nocodozole treated HeLa cell lysate. |
| Usage Statement |
|
| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at 2-8°C from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 100 µg |