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Millipore This Anti-Nrf-1 Antibody, Clone R157.1.3h3 Is Validated For Use In Western Blotting, Chromatin Immunoprecipitation (Chip) And Chip-Seq For The Detection Of Nrf-1. - Mill (Additional S&H or Hazmat Fees May Apply)
Nuclear respiratory factor 1 (UniProt Q16656; also known as Alpha-pal, Alpha palindromic-binding protein, NRF-1) is encoded by the NRF1 (also known as ALPHA-PAL) gene (Gene ID 4899) in human. NRF-1 is a redox-responsive transcription factor that plays an anti-oxidant, pro-survival role in mitochondrial biogenesis process. NRF-1 is upregulated and activated/phosphorylated by Akt in response to oxidant exposure or increased cellular reactive oxygen species (ROS), resulting in NRF-1 nuclear translocation, where it binds to DNA as a homodimer and mediates the transcription of mitochondrial transcription factor A (mtTFA) that is needed for mitochondrial DNA transcription.
Product Information
Format
Purified
Presentation
Purified mouse monoclonal IgG2aκ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality Level
MQ100
Applications
Application
This Anti-NRF-1 Antibody, clone R157.1.3H3 is validated for use in Western Blotting, Chromatin Immunoprecipitation (ChIP) and ChIP-seq for the detection of NRF-1.
Key Applications
Western Blotting
Chromatin Immunoprecipitation (ChIP)
ChIP-seq
Application Notes
Western Blotting Analysis: 1.0 µg/mL from a representative lot detected NRF-1 in 10 µg of SH-SY5Y and HeLa cell lysates. Chromatin Immunoprecipitation (ChIP) Analysis: A representative lot detected NRF-1 chromatin DNA binding activity using K562 chromatin preparations (ENCODE Data Coordination Center (DCC) accession: ENCAB000BLM). ChIP-seq Analysis: A representative lot detected NRF-1 DNA binding motifs by ChIP-seq analysis using K562 chromatin preparations (ENCODE Data Coordination Center (DCC) accession: ENCAB000BLM).
Biological Information
Immunogen
Recombinant protein corresponding to human NRF-1.
Clone
R157.1.3H3
Concentration
Please refer to lot specific datasheet.
Host
Mouse
Isotype
IgG2aκ
Species Reactivity
Human
Antibody Type
Monoclonal Antibody
Entrez Gene Number
NP_001035199
Gene Symbol
NRF1
Purification Method
Protein G Purified
UniProt Number
Q16656
Molecular Weight
~58 kDa observed. Uncharacterized band(s) may appear in some lysates.
Product Usage Statements
Quality Assurance
Evaluated by Western Blotting in Raji cell lysate.
Western Blotting Analysis: 0.5 µg/mL of this antibody detected NRF-1 in 10 µg of Raji cell lysate.
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
