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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| M | WB, FC | R | Purified | Monoclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | MABF112 |
| Description | Anti-CD70 Antibody, clone TAN 1-7 |
| Alternate Names |
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| Background Information | CD70 is an interesting membrane bound cellular factor that acts as a cytokine which binds the receptor CD27. Binding induces the proliferation and co-stimulation of T cells and enhances the generation of cytolytic T-cells. Thus, CD70 plays important roles in T cell adaptive T cell response as well as in innate immunity and is required for the proper activation of the anti-viral adaptive response. CD70 also serves as a regulatory role in early cytokine responses to pathogens through the maintenance of Treg cell survival and function. Recent research also indicates that CD70 expression could be important in autoimmune disease regulation and in treatment of autoimmune diseases such as rheumatoid arthritis. CD70 is expressed on T cells, splenocytes, and thymocytes and it belongs to the TNF family of cellular signaling proteins. |
| Product Information | |
|---|---|
| Format | Purified |
| Presentation | Purified rat monoclonal IgG2aκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mm NaCl with 0.05% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Anti-CD70 Antibody, clone TAN 1-7 | MABF112 is an antibody against CD70 Antibody for use in Western Blotting, Flow Cytometry. |
| Key Applications |
|
| Application Notes | Flow Cytometry Analysis: 2 µg of this antibody detected CD70 in mouse bone marrow dendritic cells treated with 2000U/mL GM-CSF and 0.1 ug/mL of LPS. Functional Blocking Analysis: A representative lot detected CD70 in splenic CD8a+ DCs (Arora, P., et Al. (2014). Immunity. 40:105-116). |
| Biological Information | |
|---|---|
| Immunogen | Recombinant protein corresponding to the extracellular domain of mouse CD70. |
| Epitope | Extracellular domain |
| Clone | TAN 1-7 |
| Concentration | Please refer to lot specific datasheet. |
| Host | Rat |
| Isotype | IgG2aκ |
| Species Reactivity |
|
| Antibody Type | Monoclonal Antibody |
| Entrez Gene Number |
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| Gene Symbol |
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| Purification Method | Protein g Purified |
| UniProt Number |
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| Molecular Weight | ~27 kDa observed |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Western Blotting in mouse spleen tissue lysate. Western Blotting Analysis: A 1:500 dilution of this antibody detected CD70 in 10 µg of mouse spleen tissue lysate. |
| Usage Statement |
|
| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at 2-8°C from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 100 µL |