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Millipore Anti-Cd70 Antibody, Clone Tan 1-7 | Mabf112 Is An Antibody Against Cd70 Antibody For Use In Western Blotting, Flow Cytometry. - Mill (Additional S&H or Hazmat Fees May Apply)
CD70 is an interesting membrane bound cellular factor that acts as a cytokine which binds the receptor CD27. Binding induces the proliferation and co-stimulation of T cells and enhances the generation of cytolytic T-cells. Thus, CD70 plays important roles in T cell adaptive T cell response as well as in innate immunity and is required for the proper activation of the anti-viral adaptive response. CD70 also serves as a regulatory role in early cytokine responses to pathogens through the maintenance of Treg cell survival and function. Recent research also indicates that CD70 expression could be important in autoimmune disease regulation and in treatment of autoimmune diseases such as rheumatoid arthritis. CD70 is expressed on T cells, splenocytes, and thymocytes and it belongs to the TNF family of cellular signaling proteins.
Product Information
Format
Purified
Presentation
Purified rat monoclonal IgG2aκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality Level
MQ100
Applications
Application
Anti-CD70 Antibody, clone TAN 1-7 | MABF112 is an antibody against CD70 Antibody for use in Western Blotting, Flow Cytometry.
Key Applications
Western Blotting
Flow Cytometry
Application Notes
Flow Cytometry Analysis: 2 µg of this antibody detected CD70 in mouse bone marrow dendritic cells treated with 2000U/mL GM-CSF and 0.1 ug/mL of LPS. Functional Blocking Analysis: A representative lot detected CD70 in splenic CD8a+ DCs (Arora, P., et al. (2014). Immunity. 40:105-116).
Biological Information
Immunogen
Recombinant protein corresponding to the extracellular domain of mouse CD70.
Epitope
Extracellular domain
Clone
TAN 1-7
Concentration
Please refer to lot specific datasheet.
Host
Rat
Isotype
IgG2aκ
Species Reactivity
Mouse
Antibody Type
Monoclonal Antibody
Entrez Gene Number
NP_035747
Gene Symbol
Cd70
Cd27l
Cd27lg
Tnfsf7
Purification Method
Protein G Purified
UniProt Number
O55237
Molecular Weight
~27 kDa observed
Product Usage Statements
Quality Assurance
Evaluated by Western Blotting in mouse spleen tissue lysate.
Western Blotting Analysis: A 1:500 dilution of this antibody detected CD70 in 10 µg of mouse spleen tissue lysate.
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
