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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H | FC, IHC, IP | M | Purified | Monoclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | MABF118 |
| Description | Anti-sTRAIL Antibody, clone III6F |
| Alternate Names |
|
| Background Information | TNF-related apoptosis-inducing ligand (sTRAIL or Apo-2L) is the endogenous ligand for the ubiquitous pro-apoptotic TRAIL-R1 and TRAIL-R2 receptors. Upon ligand binding, TRAIL receptors form trimeric structures at the plasma membrane around a central zinc atom. The trimeric TRAIL receptors bind the FADD adaptor protein which in turn recruits caspase 8. TRAIL/APO-2L signals ultimately converge on caspase 3 and related proteins that promote cell death. Importantly, activated TRAIL receptors work cooperatively with death receptors 4 and 5 in transformed cells to induce caspase signaling, thereby preventing the proliferation of abnormal cells. TRAIL/ApO-2L has been implicated in pathophysiology of many disease processes, including cancer and autoimmune diseases. |
| Product Information | |
|---|---|
| Format | Purified |
| Control |
|
| Presentation | Purified mouse monoclonal IgG2bκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | This Anti-sTRAIL Antibody, clone III6F is validated for use in Flow Cytometry, IHC, Immunoprecipitation for the detection of sTRAIL. |
| Key Applications |
|
| Application Notes | Immunohistochemistry Analysis: A representative lot from an independent laboratory detected sTRAIL in frozen sections from human tonsils, lymph nodes and appendix (Liabakk, N. B., et al. (2002). J Immunol Methods. 259(1-2):119-128.). Flow Cytometry Analysis: A representative lot from an independent laboratory detected sTRAIL in REH cells (Liabakk, N. B., et al. (2002). J Immunol Methods. 259(1-2):119-128.). |
| Biological Information | |
|---|---|
| Immunogen | Human soluble TRAIL |
| Clone | III6F |
| Concentration | Please refer to the Certificate of Analysis for the lot-specific concentration. |
| Host | Mouse |
| Isotype | IgG2bκ |
| Species Reactivity |
|
| Antibody Type | Monoclonal Antibody |
| Entrez Gene Number |
|
| Entrez Gene Summary | The protein encoded by this gene is a cytokine that belongs to the tumor necrosis factor (TNF) ligand family. This protein preferentially induces apoptosis in transformed and tumor cells, but does not appear to kill normal cells although it is expressed at a significant level in most normal tissues. This protein binds to several members of TNF receptor superfamily including TNFRSF10A/TRAILR1, TNFRSF10B/TRAILR2, TNFRSF10C/TRAILR3, TNFRSF10D/TRAILR4, and possibly also to TNFRSF11B/OPG. The activity of this protein may be modulated by binding to the decoy receptors TNFRSF10C/TRAILR3, TNFRSF10D/TRAILR4, and TNFRSF11B/OPG that cannot induce apoptosis. The binding of this protein to its receptors has been shown to trigger the activation of MAPK8/JNK, caspase 8, and caspase 3. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. |
| Gene Symbol |
|
| Purification Method | Protein G Purified |
| UniProt Number |
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| UniProt Summary | FUNCTION: Cytokine that binds to TNFRSF10A/TRAILR1, TNFRSF10B/TRAILR2, TNFRSF10C/TRAILR3, TNFRSF10D/TRAILR4 and possibly also to TNFRSF11B/OPG. Induces apoptosis. Its activity may be modulated by binding to the decoy receptors TNFRSF10C/TRAILR3, TNFRSF10D/TRAILR4 and TNFRSF11B/OPG that cannot induce apoptosis. COFACTOR: Binds 1 zinc ion per trimer. SUBUNIT STRUCTURE: Homotrimer. SUBCELLULAR LOCATION: Membrane; Single-pass type II membrane protein. TISSUE SPECIFICITY: Widespread; most predominant in spleen, lung and prostate. SEQUENCE SIMILARITIES: Belongs to the tumor necrosis factor family. |
| Molecular Weight | 33 kDa calculated |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Flow Cytometry in HeLa cells. Flow Cytometry Analysis: 2.5 µg/mL of this antibody detected sTRAIL in 2X10E5 HeLa cells. |
| Usage Statement |
|
| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at 2-8°C from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 100 µg |