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Millipore This Armenian Hamster Monoclonal Anti-Tcr Beta Chain Antibody (Mouse), Violetfluor™ 450, Clone H57-597, Cat. No. Mabf1459 Is Validated For Use In Flow Cytometry, For The - Mill (Additional S&H or Hazmat Fees May Apply)
The H57-597 antibody is specific for the beta chain of the mouse T cell Receptor (TCR). This cell surface protein combines with a second protein chain (alpha chain) to form the alpha-beta TCR that is expressed by NK1.1+ thymocytes, NKT cells, and the majority of peripheral T cells. A small number of T cells may express an alternative heteromer of gamma/delta protein chains, known as the g/d TCR. These receptors participate in a complex with CD3, and with the co-receptors CD4 or CD8, to recognize and respond to antigens bound to MHC molecules on antigen-presenting cells. Such interactions promote T cell receptor signaling (T cell activation) and can result in a number of cellular responses including proliferation, differentiation, production of cytokines or activation-induced cell death.The H57-597 antibody is used as a phenotypic marker for T cells expressing the alpha-beta TCR. It is also widely used to cross-link surface TCR and thereby mimic TCR-mediated cell activation or induction of apoptosis. The antibody does not cross-react with cells expressing the g/d TCR.
Product Information
Format
violetFluor™ 450
Presentation
Purified Armenian Hamster monoclonal containing 10 mM NaH2PO4, 150 mM NaCl, 0.09% NaN3, 0.1% gelatin, pH7.2.
Quality Level
MQ100
Applications
Application
This armenian hamster monoclonal Anti-TCR beta chain Antibody (mouse), violetFluor™ 450, clone H57-597, Cat. No. MABF1459 is validated for use in Flow Cytometry, for the detection of TCR beta chain.
Key Applications
Flow Cytometry
Biological Information
Immunogen
Immunogen information for this antibody is proprietary information.
Clone
H57-597
Concentration
Please refer to lot specific datasheet.
Host
Armenian Hamster
Isotype
IgG
Species Reactivity
Mouse
Antibody Type
Monoclonal Antibody
Gene Symbol
Immunoglobulins & Immunology
Purification Method
Purified by size-exclusion chromatography
UniProt Number
P0C6B2
Product Usage Statements
Quality Assurance
This monoclonal antibody was purified from tissue culture supernatant via affinity chromatography. The purified antibody was conjugated under optimal conditions, with unreacted dye removed from the preparation.
Flow Cytometry Analysis: 0.25 μg from a representative lot detected TCR beta chain in one million C57Bl/6 splenocytes.
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions
Stable for 1 year at 2-8°C from date of receipt. Note: It is recommended to store the product undiluted at 2-8°C and protected from prolonged exposure to light. Do not freeze.
