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Millipore This Rat Monoclonal Anti-Cd45 Antibody (Mouse), Pe-Cy7, Clone 30-F11, Cat. No. Mabf1465 Is Validated For Use In Flow Cytometry, For The Detection Of Cd45. - Mill (Additional S&H or Hazmat Fees May Apply)
The 30-F11 antibody reacts with mouse CD45, which is one of the most abundant hematopoietic markers and is expressed on all leukocytes (the Leukocyte Common Antigen, LCA). CD45 is a protein tyrosine phosphatase existing in several isoforms, each being generated and expressed in cell-specific patterns. With its broad cell distribution, CD45 is critical for many leukocyte functions, regulating signal transduction and cell activation associated with the T cell receptor, B cell receptor, and IL-2 receptor. Other forms of CD45, with restricted cellular expression, include CD45R (B220), CD45RA, CD45RB, CD45RO and others. The 30-F11 antibody is widely used as a leukocyte marker for B cells, T cell subsets and NK cell subsets.
Product Information
Format
PE-Cy7
Presentation
Purified Rat monoclonal containing 10 mM NaH2PO4, 150 mM NaCl, 0.09% NaN3, 0.1% gelatin, pH7.2.
Quality Level
MQ100
Applications
Application
This rat monoclonal Anti-CD45 Antibody (Mouse), PE-Cy7, clone 30-F11, Cat. No. MABF1465 is validated for use in Flow Cytometry, for the detection of CD45.
Key Applications
Flow Cytometry
Biological Information
Immunogen
Immunogen information for this antibody is proprietary information.
Clone
30-F11
Concentration
Please refer to lot specific datasheet.
Host
Rat
Isotype
IgG2bκ
Species Reactivity
Mouse
Antibody Type
Monoclonal Antibody
Gene Symbol
Ptprc
Ly-5
Purification Method
Purified by size-exclusion chromatography
UniProt Number
P06800
Product Usage Statements
Quality Assurance
This monoclonal antibody was purified from tissue culture supernatant via affinity chromatography. The purified antibody was conjugated under optimal conditions, with unreacted dye removed from the preparation.
Flow Cytometry Analysis: 0.125 μg from a representative lot detected CD45 in one million C57Bl/6 splenocytes.
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions
Stable for 1 year at 2-8°C from date of receipt. Note: It is recommended to store the product undiluted at 2-8°C and protected from prolonged exposure to light. Do not freeze.
