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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| M | FC, ACT, IP | M | Purified | Monoclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | MABF1495Z |
| Description | Anti-NK1.1 (mouse) Antibody, clone PK136, Azide Free |
| Alternate Names |
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| Background Information | The NK cell receptor protein 1 (NKR-P1), a.k.a. CD161, molecules represent a family of type II transmembrane C-type lectin-like receptors expressed predominantly by NK cells. A total of five murine NKR-P1-coding genes (Klrb1a/b/c/d/f) have been identified, with NKR-P1A/C/F (CD161a/c/f) functioning as stimulatory receptors that possess a charged transmembrane arginine esidue thought to be important for association with the FcRγ adaptor protein, and NKR-P1B/D as two inhibitory receptors that possess a consensus cytoplasmic ITIM (L/VxYxxL/I/V) motif. The ITIM motif is shown to mediate NKR-P1B recruitment of Src homology 2 (SH2)-containing protein tyrosine phosphatase-1 (SHP-1) in a phosphorylation-dependent manner. All murine NKR-P1 proteins possess a Cys-X-Cys-Pro (CxCP) motif that mediates association with the Src-related nonreceptor protein tyrosine kinase p56lck. Both NKR-P1B and NKR-P1C functionally associate with p56lck, while mutation of the CxCP motif abolishes NKR-P1B/C-mediated signal transduction. Likewise, mutation of ITIM motif abolishes NKR-P1B SHP-1 association as well as NKR-P1B-dependent inhibitory signaling. |
| Product Information | |
|---|---|
| Format | Purified |
| Presentation | Purified mouse monoclonal IgG2aκ antibody in PBS without preservatives. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Anti-NK1.1 (mouse) Antibody, clone PK136, Azide Free is an antibody against NK1.1 (mouse) for use in Flow Cytometry, Activity Assay, Immunohistochemistry, Immunoprecipitation. |
| Key Applications |
|
| Application Notes | Activity Assay: Representative lots, when administered to mice via i.p. injection, depleted splenic natural killer (NK) cell activity in a dose-dependent manner (Xiao, T.Z., et Al. (2012). Biol. Blood Marrow Transplant. 18(2):200-209; Seaman, W.E., et Al. (1987). J. Immunol. 138(12):4539-4544). Activity Assay: A representative lot activated target cell killing by cross-linking NK cell surface NK1.1 (NKR-P1C) and target cell surface Fc receptor. Ab-induced redirected lysis (AIRL) assay employing NK cells expressing both NKR-P1B and NKR-P1C prevented NK cell cytotoxicity activation by clone PK136 (Carlyle, J.R., et Al. (1999). J. Immunol. 162(10):5917-5923). Immunoprecipitation Analysis: A representative lot co-immunoprecipitated SHP-1, but not SHP-2 or SHIP, from Swiss NIH mouse-derived MNK-1 pre-NK cells upon upregulating NKR-P1B intracellular ITIM motif tyrosine phosphorylation by pervanadate treatment (Carlyle, J.R., et Al. (1999). J. Immunol. 162(10):5917-5923). Flow Cytometry Analysis: A representative lot immunostained the surface of Jurkat transfectants expressing C57BL/6J (B6) mouse-derived NKR-P1B/CD161b or Swiss NIH (Sw) mouse-derived NKR-P1C/CD161c/NK1.1, but not Jurkat transfectants expressing B6-derived NKR-P1A/CD161a (Carlyle, J.R., et Al. (1999). J. Immunol. 162(10):5917-5923). Flow Cytometry Analysis: A representative lot immunostained NKR-P1B/CD161b-expressing NK cells from Swiss NIH (Sw) mouse strain, NKR-P1C/CD161c/NK1.1-expressing NK cells from C57BL/6J (B6), as well as (B6 3 Sw)F1 cross-strain-derived NK cells expressing both NKR-P1B and NKR-P1C (Carlyle, J.R., et Al. (1999). J. Immunol. 162(10):5917-5923). Note: Clone PK136 is also available in the following conjugated forms for flow cytometry application, APC (MABF1487 & MABF1488), FITC (MABF1489 & MABF1490), PE (MABF1491), PerCP-Cy5.5 (MABF1493 & MABF1494). redFluor™ 710 (MABF1496 & MABF1497). |
| Biological Information | |
|---|---|
| Immunogen | Mouse spleen and bone marrow cells (Koo, g.C., and Peppard, J.R. (1984). Hybridoma. 3(3):301-303). |
| Epitope | Extracellular domain. |
| Clone | PK136 |
| Concentration | Please refer to lot specific datasheet. |
| Host | Mouse |
| Specificity | Clone PK136 recognizes murine NKR-P1B/CD161b (UniProt P27812) and NK1.1/NKR-P1C/CD161c (UniProt P27814), but not CD161a/NKR-P1A (P27811). Upon activation by PK136 binding, NKR-P1C/NK1.1 signals to activate, while NKR-P1B signals to suppress, NK cells for cytotoxic killing of target cells (Carlyle, J.R., et Al. (1999). J. Immunol. 162(10):5917-5923). Epitope is present in both spliced isoforms of murine NK1.1/CD161c reported by UniProt (P27814). Clone PK136 does not react with CD161a (by Klrb1a gene) or C57BL/6 mouse strain-derived CDC161b variant (by Klrb1b gene, also known as Klrb1d). There exists a fifth murine CD161 gene Klrb1f, reactivity of this mAb toward this gene product has not been established. |
| Isotype | IgG2aκ |
| Species Reactivity |
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| Antibody Type | Monoclonal Antibody |
| Entrez Gene Number |
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| Gene Symbol |
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| Purification Method | Protein g purified. |
| UniProt Number |
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| Molecular Weight | 25.08 kDa (NK1.1/CD161c isoform 1) and 24.77 kDa (NK1.1/CD161c isoform 2) calculated. |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Flow Cytometry in mouse splenocytes. Flow Cytometry Analysis: 1.0 µg of this antibody detected NK1.1-positive mouse splenocytes. |
| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. |
| Packaging Information | |
|---|---|
| Material Size | 100 μg |