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Millipore This Rat Monoclonal Anti-Cd8a Antibody (Mouse), Fitc, Clone 53-6.7, Cat. No. Mabf1541 Is Validated For Use In Flow Cytometry, For The Detection Of Cd8a. - Mill (Additional S&H or Hazmat Fees May Apply)
The 53-6.7 antibody reacts with the 32-34 kDa alpha subunit of mouse CD8, known as CD8a or CD8 alpha. CD8a can form a homodimer (CD8 alpha-alpha), but is more commonly expressed as a heterodimer with a second chain known as CD8b or CD8 beta. CD8 acts as a co-receptor in antigen recognition and subsequent T cell activation that is initiated upon binding of the T cell receptor (TCR) to antigen-bearing MHC Class I molecules. The cytoplasmic domains of CD8 provide binding sites for the tyrosine kinase lck, facilitating intracellular signaling events that lead to T cell activation, development, and cytotoxic effector functions. CD8+ cytotoxic T cells (CTLs) play an important role in inducing cell death of tumor cells, as well as cells infected by virus, bacteria or parasites.The 53-6.7 antibody is widely used as a phenotypic marker for mouse CD8a expression on cytotoxic T cells, thymocytes, as well as on certain cell types that do not also express the TCR, including some NK cells and lymphoid dendritic cells.
Product Information
Format
FITC
Presentation
Purified Rat monoclonal containing 10 mM NaH2PO4, 150 mM NaCl, 0.09% NaN3, 0.1% gelatin, pH7.2.
Quality Level
MQ100
Applications
Application
This rat monoclonal Anti-CD8a Antibody (Mouse), FITC, clone 53-6.7, Cat. No. MABF1541 is validated for use in Flow Cytometry, for the detection of CD8a.
Key Applications
Flow Cytometry
Biological Information
Immunogen
Immunogen information for this antibody is proprietary information.
Clone
53-6.7
Concentration
Please refer to lot specific datasheet.
Host
Rat
Isotype
IgG2aκ
Species Reactivity
Mouse
Antibody Type
Monoclonal Antibody
Gene Symbol
CD8a
Purification Method
Purified by size-exclusion chromatography
UniProt Number
P01731
Product Usage Statements
Quality Assurance
This monoclonal antibody was purified from tissue culture supernatant via affinity chromatography. The purified antibody was conjugated under optimal conditions, with unreacted dye removed from the preparation.
Flow Cytometry Analysis: 0.5 μg from a representative lot detected CD8a in one million C57Bl/6 splenocytes.
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions
Stable for 1 year at 2-8°C from date of receipt. Note: It is recommended to store the product undiluted at 2-8°C and protected from prolonged exposure to light. Do not freeze.
