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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H | FC | M | FITC | Monoclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | MABF1675 |
| Description | Anti-CD8a (human), FITC, clone RPA-T8 Antibody |
| Alternate Names |
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| Background Information | The RPA-T8 antibody is specific for the 32-34 kDa alpha chain of human CD8, known as CD8a or CD8 alpha. CD8a can form a homodimer (CD8 alpha-alpha), but is more commonly expressed as a heterodimer with a second chain known as CD8b or CD8 beta. CD8 acts as a co-receptor for antigen recognition and subsequent T cell activation that is initiated upon binding of the T cell receptor (TCR) to antigen-bearing MHC Class I molecules. The cytoplasmic domains of CD8 provide binding sites for the tyrosine kinase lck, facilitating intracellular signaling events that lead to T cell activation, development, and cytotoxic effector functions. CD8+ cytotoxic T cells (CTLs) play an important role in inducing cell death of tumor cells, as well as cells infected by virus, bacteria or parasites.The RPA-T8 antibody is widely used as a phenotypic marker for CD8 on cytotoxic T cells, thymocytes, as well as on certain cell types that do not also express the TCR, including some NK cells and lymphoid dendritic cells. It is cross-reactive with CD8 in several non-human species, including Baboon, Chimpanzee, Cynomolgus and Rhesus. If used together with an alternative Anti-Human CD8a clone, Hit8a, the RPA-T8 antibody will not block binding of Hit8a to CD8a. |
| Product Information | |
|---|---|
| Format | FITC |
| Presentation | Purified Mouse monoclonal containing 10 mm NaH2PO4, 150 mm NaCl, 0.09% NaN3, 0.1% gelatin, pH7.2. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Anti-CD8a (human), FITC, clone RPA-T8 Antibody is an antibody against CD8a (human), FITC, clone RPA-T8 for use in Flow Cytometry. |
| Key Applications |
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| Biological Information | |
|---|---|
| Immunogen | Immunogen information for this antibody is proprietary information. |
| Clone | RPA-T8 |
| Concentration | Please refer to lot specific datasheet. |
| Host | Mouse |
| Isotype | IgG1κ |
| Species Reactivity |
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| Antibody Type | Monoclonal Antibody |
| Gene Symbol |
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| Purification Method | Purified by size-exclusion chromatography |
| UniProt Number |
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| Product Usage Statements | |
|---|---|
| Quality Assurance | This monoclonal antibody was purified from tissue culture supernatant via affinity chromatography. The purified antibody was conjugated under optimal conditions, with unreacted dye removed from the preparation. Flow Cytometry Analysis: 1 μg from a representative lot detected CD8a in one million human peripheral blood lymphocytes. |
| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at 2-8°C from date of receipt. Note: It is recommended to store the product undiluted at 2-8°C and protected from prolonged exposure to light. Do not freeze. |
| Packaging Information | |
|---|---|
| Material Size | 500 tests |