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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H, M | WB, IHC | R | Purified | Monoclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | MABF267 |
| Description | Anti-Cochlin Antibody/COCH, clone 9A10D2 |
| Alternate Names |
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| Background Information | The protein Cochlin/COCH is important in the development of the cochlea and vestibule in mammals as well as other functions. Cochlin/COCH is an extracellular matrix protein that plays a role in cell shape, positioning and motility. Recent research shows that it is also important in our innate immune response to bacteria and pathogens. Upon bacterial infection, a fragment of Cochlin/COCH is released from the conduits of B cell follicles in the spleen and this triggers protective cytokines in the periphery. Cochlin/COCH is expressed in epithelial tissues in various tissues, particularly in follicular cells and network of conduits in the spleen and lymph nodes. Cochlin/COCH also is a target for BMP4 mediation in embryonic stem cells (ESCs) where it facilitates self-renewal. The protein also appears to suppress neural differentiation of ESCs and suggests that Cochlin/COCH protein is important in ES cell fate determination. Mutations in the Cochlin/COCH gene are responsible for human DFNA9 syndrome, a disorder characterized by neurodegeneration of the inner ear that leads to hearing loss and vestibular impairments. |
| Product Information | |
|---|---|
| Format | Purified |
| Presentation | Purified rat monoclonal IgG2aκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | This Anti-Cochlin Antibody/COCH, clone 9A10D2 is validated for use in Western Blotting and Immunohistochemistry for the detection of Cochlin/COCH. |
| Key Applications |
|
| Application Notes | Western Blotting Analysis: 1.0 µg/mL from a representative lot detected Cochlin/COCH in 10 µg of human lymph node tissue lysate. Western Blotting Analysis: A representative lot detected Cochlin/COCH in spleen and lymph node from WT and COCH knockout mice (Py, B.F., et al. (2013). Immunity. 38:1063-1072). Western Blotting Analysis: A representative lot detected Cochlin/COCH in protein cleaved into p18 and p8 products. (Py, B.F., et al. (2013). Immunity. 38:1063-1072). Immunohistochemistry Analysis: A 1:50 dilution from a representative lot detected Cochlin/COCH in mouse spleen tissue. Immunohistochemistry Analysis: A representative lot detected Cochlin/COCH in spleen and lymphnode from WT and Coch knockout mice (Py, B.F., et al. (2013). Immunity. 38:1063-1072). |
| Biological Information | |
|---|---|
| Immunogen | Recombinant protein corresponding to human Cochlin/COCH. |
| Clone | 9A10D2 |
| Concentration | Please refer to lot specific datasheet. |
| Host | Rat |
| Isotype | IgG2aκ |
| Species Reactivity |
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| Antibody Type | Monoclonal Antibody |
| Entrez Gene Number |
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| Gene Symbol |
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| Purification Method | Protein G Purified |
| UniProt Number |
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| Molecular Weight | ~59 kDa observed. This protein is cleaved into p18 and p8 products. Uncharacterized bands may be observed in some lysates. |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Western Blotting in mouse spleen tissue lysate. Western Blotting Analysis: 1.0 µg/mL of this antibody detected Cochlin/COCH in 10 µg of mouse spleen tissue lysate. |
| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at 2-8°C from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 100 µg |