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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| M, H | FC, Enzyme Assays | M | Purified | Monoclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | MABF28 |
| Replaces | MABS174 |
| Description | Anti-PAR-3 Antibody, clone 8E8 |
| Alternate Names |
|
| Background Information | PAR-3 (also known as Coagulation factor II receptor-like 2, Thrombin receptor-like 2 or F2RL2) is an adapter protein involved in asymmetrical cell division and cell polarization processes. It seems to play a central role in the formation of epithelial tight junctions. Its association with PARD6B may prevent the interaction of PAR-3 with F11R/JAM1, thereby preventing tight junction assembly. The PARD6-PAR-3 complex links GTP bound Rho small GTPases to atypical protein kinase C proteins. PAR-3 interacts with PARD6A and PARD6B. Isoform 2, but not at least isoform 3 interacts with PRKCZ. PAR-3 interacts with PRCKI. PAR-3 forms part of a complex with PARD6A or PARD6B, PRKCI or PRKCZ and CDC42 or RAC1. PAR-3 interacts with F11R/JAM1. Antibodies against PAR-3 are present in sera from patients with cutaneous T cell lymphomas. |
| Product Information | |
|---|---|
| Format | Purified |
| Control |
|
| Presentation | Purified mouse monoclonal IgG2bκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Anti-PAR-3 Antibody, clone 8E8 detects level of PAR-3 & has been published & validated for use in FC, EA. |
| Key Applications |
|
| Application Notes | Flow Cytometry Analysis: A previous lot was used by an independent laboratory in FC. (Petrova, Y., et al. (2008). Centr Eur J Immunol. 33 (1):14-18.) Activity Assay Analysis (platelet aggregation): A previous lot was used by an independent laboratory in platelet aggregation assay. (Petrova, Y., et al. (2008). Centr Eur J Immunol. 33 (1):14-18.) |
| Biological Information | |
|---|---|
| Immunogen | KLH-conjugated linear peptide corresponding to human PAR-3. |
| Clone | 8E8 |
| Concentration | Please refer to the Certificate of Analysis for the lot-specific concentration. |
| Host | Mouse |
| Isotype | IgG2bκ |
| Species Reactivity |
|
| Species Reactivity Note | Demonstrated to react with mouse. Predicted to react with human based on 100% sequence homology. |
| Antibody Type | Monoclonal Antibody |
| Entrez Gene Number |
|
| Entrez Gene Summary | Coagulation factor II (thrombin) receptor-like 2 (F2RL2) is a member of the large family of 7-transmembrane-region receptors that couple to guanosine-nucleotide-binding proteins. F2RL2 is also a member of the protease-activated receptor family and activated by thrombin. F2RL2 is activated by proteolytic cleavage of its extracellular amino terminus. The new amino terminus functions as a tethered ligand and activates the receptor. F2RL2 is a cofactor for F2RL3 activation by thrombin. It mediates thrombin-triggered phosphoinositide hydrolysis and is expressed in a variety of tissues. [provided by RefSeq]. |
| Gene Symbol |
|
| Purification Method | Protein G Purified |
| UniProt Number |
|
| UniProt Summary | FUNCTION: Receptor for activated thrombin coupled to G proteins that stimulate phosphoinositide hydrolysis. SUBUNIT STRUCTURE: Interacts with INSC/inscuteable and probably GPSM2. SUBCELLULAR LOCATION: Cell membrane; Multi-pass membrane protein. TISSUE SPECIFICTY: Highest expression in the megakaryocytes of the bone marrow, lower in mature megakaryocytes, in platelets and in a variety of other tissues such as heart and gut. PTM: A proteolytic cleavage generates a new N-terminus that functions as a tethered ligand. SEQUENCE SIMILARITIES: Belongs to the G-protein coupled receptor 1 family. SEQUENCE CAUTION: The sequence CAD97628.1 differs from that shown. Reason: Frameshift at position 374. |
| Molecular Weight | 40 kDa calculated |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Flow Cytometry in mouse platelets from washed whole blood (heparinized). Flow Cytometry Analysis: 2 µg of this antibody detected PAR-3 in mouse platelets from washed whole blood (heparinized). |
| Usage Statement |
|
| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at 2-8°C from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 100 µg |