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Millipore This Anti-Roquin-1/2, Clone 3f12 Antibody Is Validated For Use In Western Blotting And Immunoprecipitation For The Detection Of Roquin-1/2. - Mill (Additional S&H or Hazmat Fees May Apply)
RING finger and CCCH-type zinc finger domain-containing protein 1
RING finger protein 198
Background Information
Roquin-1 shares 46% identity with Roquin-2 and both serve the same function and belong to the Zinc finger family of RNA binding proteins. Roquin-1/2 are cytoplasmic RNA suppression proteins that specifically bind selective RNAs and promote the deadenylation of mature mRNAs and their degradation. Roquin-1/2s targets include HMGXB3, ICOS, IER3, NFKBID, NFKBIZ, PPP1R10, TNF and in many more critical RNAs. In particular, because of their impact on ICSO and gamma interferon and T helper cell activation, Roquin-1/2 are being examined as mediators of the systemic inflammation response and controllers of adaptive and innate immune responses in autoimmune disease. Roquin 1/2 are widely expressed and localized in the cytoplasm and/or P bodies and cytosolic stress granules when under cellular stress.
Product Information
Format
Purified
Presentation
Purified rat monoclonal IgG2aκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality Level
MQ100
Applications
Application
This Anti-Roquin-1/2, clone 3F12 Antibody is validated for use in Western Blotting and Immunoprecipitation for the detection of Roquin-1/2.
Key Applications
Western Blotting
Immunoprecipitation
Application Notes
Western Blotting Analysis: A representative lot detected Roquin-1/2 in both Roquin-1 & Roquin-2 in normal MEFs, only Roquin-2 and Roquin-1 deficient MEFs, and neither protein in Roquin-1/2 deficient MEFs. This antibody also detected Roquin-1 and Roquin-2 in Mouse Spleen, Lymph Node, Thymus, Brain & Lung tissue, CD4+ T cells and Thymocyte lysates (Vogel, K.U., et al. (2013). Immunity. 38:655-668). Immunoprecipitation Analysis: A representative lot immunoprecipitated Roquin-1/2 in MEF cell lysate (Vogel, K.U., et al. (2013). Immunity. 38:655-668).
Biological Information
Immunogen
Recombinant protein corresponding to human Roquin-1/2.
Clone
3F12
Concentration
Please refer to lot specific datasheet.
Host
Rat
Specificity
This antibody specifically detects both Roquin-1 & Roquin-2 (Vogel, K.U., et al. (2013). Immunity. 38:655-668).
Isotype
IgG2aκ
Species Reactivity
Human
Mouse
Antibody Type
Monoclonal Antibody
Entrez Gene Number
NP_742068
Gene Symbol
RC3H1
KIAA2025
RNF198
Purification Method
Protein G Purified
UniProt Number
Q5TC82
Molecular Weight
125 kDa calculated
Product Usage Statements
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
