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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H | IHC, WB | M | Purified | Monoclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | MABF31 |
| Description | Anti-Alpha Defensin-5 (HD5) Antibody, clone 8C8 |
| Alternate Names |
|
| Background Information | Human alpha defensin-5 (HD5) is one of six peptides belonging to the alpha defensin family. Defensins are small peptides involved in immunological defense against viral and microbial infections through either direct interaction with pathogens or in combination with other immune system factors. There are three subfamilies of defensins (α, β, and θ). α defensins are only observed in mammals and are mainly β-sheet structures. Alpha defensin-5 (HD5) is primarily expressed in the Paneth cells found within the small intestine, and has been shown to possess activity against both Gram-positive and Gram-negative microbes. Studies have shown alpha defensin-5 (HD5) to be a strong antagonist towards human Papillomavirus infection. It may also play a role Crohn’s disease. |
| Product Information | |
|---|---|
| Format | Purified |
| Control |
|
| Presentation | Purified mouse monoclonal IgG2bκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Anti-Alpha Defensin-5 (HD5) Antibody, clone 8C8 detects level of Alpha Defensin-5 (HD5) & has been published & validated for use in IH & WB. |
| Key Applications |
|
| Application Notes | Western Blot Analysis: 0.5 ug/mL from a previous lot detected alpha defensin-5 (HD5) in human small intestine tissue lysate. The urea-treated method is recommended instead of SDS page (Shen, B. et al., 2005). |
| Biological Information | |
|---|---|
| Immunogen | Recombinant protein corresponding to human alpha defensin-5 (HD5). |
| Clone | 8C8 |
| Concentration | Please refer to the Certificate of Analysis for the lot-specific concentration. |
| Host | Mouse |
| Isotype | IgG2bκ |
| Species Reactivity |
|
| Antibody Type | Monoclonal Antibody |
| Entrez Gene Number |
|
| Entrez Gene Summary | Defensins are a family of microbicidal and cytotoxic peptides thought to be involved in host defense. They are abundant in the granules of neutrophils and also found in the epithelia of mucosal surfaces such as those of the intestine, respiratory tract, urinary tract, and vagina. Members of the defensin family are highly similar in protein sequence and distinguished by a conserved cysteine motif. Several of the alpha defensin genes appear to be clustered on chromosome 8. The protein encoded by this gene, defensin, alpha 5, is highly expressed in the secretory granules of Paneth cells of the ileum. [provided by RefSeq]. |
| Gene Symbol |
|
| Purification Method | Protein G Purified |
| UniProt Number |
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| UniProt Summary | FUNCTION: Has antimicrobial activity against Gram-negative and Gram-positive bacteria. Defensins are thought to kill microbes by permeabilizing their plasma membrane. SUBUNIT STRUCTURE: Homodimer. SUBCELLULAR LOCATION: Secreted. TISSUE SPECIFICTY: Paneth cells of the small intestine. SEQUENCE SIMILARITIES: Belongs to the alpha-defensin family. |
| Molecular Weight | 10 kDa calculated |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Immunohistochemistry in normal human tranverse colon tissue. Immunohistochemistry Analysis: A 1:200 dilution of this antibody detected alpha defensin-5 (HD5) in normal human tranverse colon tissue. |
| Usage Statement |
|
| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at 2-8°C from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 100 µg |