The store will not work correctly when cookies are disabled.
We use cookies to make your experience better.To comply with the new e-Privacy directive, we need to ask for your consent to set the cookies. Learn more.
NETA ALERT: Due to inclement weather our offices are closed today, Monday Feb. 23. We will not be shipping orders.
Millipore Anti-Thy-1 Antibody, Clone M5/49 Detects Level Of Thy-1 & Has Been Published & Validated For Use In Wb. - Mill (Additional S&H or Hazmat Fees May Apply)
Thy-1 membrane glycoprotein (Thy-1) is a glycosylphosphatidylinositol (GPI)-anchored surface protein belonging to the immunoglobulin superfamily and is a major cell surface protein found on both T–cells and in the brain. Thy-1 is thought to be involved in interactions taking place during synaptogenesis and other neurological events between cells or cells and their ligands. There are two known alleles for Thy-1; Thy1.1 and Thy1.2. Studies have shown N-glycosolation to take place at three locations on the protein.
Product Information
Format
Purified
Control
P388D1 cell lysate
Presentation
Purified rat monoclonal IgG2aκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality Level
MQ100
Applications
Application
Anti-Thy-1 Antibody, clone M5/49 detects level of Thy-1 & has been published & validated for use in WB.
Key Applications
Western Blotting
Affects Function
Application Notes
Functional Assay: A previous lot of this antibody was used for the inhibition of B6 Anti-BNLΦ CTL mediated cell death. (Davignon, D., et al. (1981). PNAS USA. 78(7):4535-4539.)
Biological Information
Immunogen
B6 Anti-BNL4Φ lymphocytes
Clone
M549.4.1
Concentration
Please refer to the Certificate of Analysis for the lot-specific concentration.
Host
Rat
Isotype
IgG2aκ
Species Reactivity
Mouse
Antibody Type
Monoclonal Antibody
Entrez Gene Number
NP_033408
Gene Symbol
THY1
CD90
Thy-1
CDw90
Purification Method
Protein G Purified
UniProt Number
P01831
UniProt Summary
FUNCTION: May play a role in cell-cell or cell-ligand interactions during synaptogenesis and other events in the brain.
~22 kDa observed. Higher bands may be observed due to glycosylation.
Product Usage Statements
Quality Assurance
Evaluated by Western Blot in P388D1 cell lysate.
Western Blot Analysis: 0.5 µg/mL of this antibody detected Thy-1 in 10 µg of P388D1 cell lysate.
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
