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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| M, H | WB, FC, ICC, IP | M | Purified | Monoclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | MABF39-I |
| Description | Anti-DC-STAMP Antibody, clone 1A2 |
| Alternate Names |
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| Background Information | Dendritic cell-specific transmembrane protein (UniProt Q9H295; also known as DC-STAMP, Dendrocyte-expressed seven transmembrane protein, FIND, hDC-STAMP, IL-four-induced protein, Transmembrane 7 superfamily member 4) is encoded by the DCSTAMP (also known as TM7SF4) gene (Gene ID 81501) in human. DC-STAMP is a six-transmembrane protein essential for cell-to-cell fusion to form multinucleated osteoclasts (OCs) during osteoclastogenesis. DC-STAMP expression is upregulated among osteoclast precursor (OCP) cells upon exposure to OC-promoting cytokines, such as receptor activator of nuclear factor-κB (NF-κB) ligand (RANKL), and Dcstamp-knockout (KO) mice have few multinucleated TRAP+ OCs and increased bone mass. On the other hand, DC-STAMP overexpression in transgenic (Tg) mice causes accelerated cell-to-cell fusion during OCP differentiation and enhanced bone resorption. DC-STAMP is a six-trasmembrane (a.a. 35-55, 58-78. 98-118, 210-230, 293-313, 377-397) protein, having both its N- and C-terminal ends exposed intracellularly (a.a. 1-34, 398-470). The C-terminal cytoplasmic tail of DC-STAMP contains an immunoreceptor tyrosine-based inhibitory motif or ITIM sequence (407-SFYPSV-412) that, when phosphorylated on the tyrosine residue, recruits SHP-1. DC-STAMP neutralizing antibody blocks OC formation in vitro and abolishes cellular DC-STAMP and SHP-1 tyrosine phosphorylation. |
| Product Information | |
|---|---|
| Format | Purified |
| Presentation | Purified mouse monoclonal IgG2aκ antibody in PBS without preservatives. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Anti-DC-STAMP Antibody, clone 1A2 is an antibody against DC-STAMP for use in Western Blotting, Flow Cytometry, Immunocytochemistry, Immunoprecipitation. |
| Key Applications |
|
| Application Notes | Western Blotting Analysis: 4.0 µg/mL from a representative lot detected DC-STAMP in 10 µg of thymus lysates from wild-type, but not Dcstamp-knockout mice. Western Blotting Analysis: 1.0 µg/mL from a representative lot detected DC-STAMP in thymus and spleen lysates from wild-type mice, and greatly reduced DC-STAMP in thymus and spleen lysates from Dcstamp-knockout mice (Courtesy of Grace Chiu, Ph.D., University of Rochester Medical Center, NY, USA). Western Blotting Analysis: A representative lot detected the ~106 kDa dimeric and the ~53 kDa monomeric DC-STAMP band by Western blotting under non-denatured and denatured condition, respectively, following DC-STAMP immunoprecipitation using murine RAW 264.7 macrophage lysate (Mensah, K.A., et al. (2010). J. Cell. Physiol. 223(1):76-83). Flow Cytometry Analysis: A representative lot detected DC-STAMP-positive lymphoctes and monocytes in purified human PBMCs (Chiu, Y.H., et al. (2012). J. Bone Miner. Res. 27(1):79-92). Flow Cytometry Analysis: A representative lot detected DC-STAMP surface expression on murine RAW 264.7 macrophages and murine bone marrow-derived CD11b+ monocytes. DC-STAMP is expressed on osteoclast precursor (OCP) cells as a dimer, which is efficiently detected by flow cytometry using clone 1A2 (Mensah, K.A., et al. (2010). J. Cell. Physiol. 223(1):76-83). Function Analysis: A representative lot inhibited RANKL & M-CSF treatment-induced osteoclasts (OC) formation in human PBMCs & monocytes cultures (Chiu, Y.H., et al. (2012). J. Bone Miner. Res. 27(1):79-92). Function Analysis: A representative lot inhibited RANKL treatment-induced osteoclasts (OC) formation in RAW 264.7 and murine bone marrow macrophages cultures (Mensah, K.A., et al. (2010). J. Cell. Physiol. 223(1):76-83). Immunohistochemistry Analysis: A representative lot detected DC-STAMP expressionon in multinucleated ‘osteoclast-like’ giant cells in human giant cell tumor of bone (Chiu, Y.H., et al. (2012). J. Bone Miner. Res. 27(1):79-92). Immunocytochemistry Analysis: A representative lot detected DC-STAMP-positive human PBMCs using 10% NBF-fixed, paraffin-embedded cell preparation (Chiu, Y.H., et al. (2012). J. Bone Miner. Res. 27(1):79-92). Immunocytochemistry Analysis: A representative lot detected differential DC-STAMP intracellular localizations by fluorescent immunocytochemistry staining of 4% paraformaldehyde-fixed, 0.1% saponin-permeabilized murine bone marrow macrophages at different time points during osteoclastogenesis upon RANKL treatment (Mensah, K.A., et al. (2010). J. Cell. Physiol. 223(1):76-83). Immunoprecipitation Analysis: A representative lot immunoprecipitated DC-STAMP from the lysates of human monocytes (Chiu, Y.H., et al. (2012). J. Bone Miner. Res. 27(1):79-92). Immunoprecipitation Analysis: A representative lot immunoprecipitated DC-STAMP from the membrane extracts of murine RAW 264.7 macrophages (Mensah, K.A., et al. (2010). J. Cell. Physiol. 223(1):76-83). |
| Biological Information | |
|---|---|
| Immunogen | KLH-conjugated linear peptide corresponding a sequence from the third extracellular domain of human DC-STAMP. |
| Epitope | The third extracellular domain. |
| Clone | 1A2 |
| Concentration | Please refer to lot specific datasheet. |
| Host | Mouse |
| Specificity | Clone 1A2 recognizes an epitope conserved between human and murine DC-STAMP in the third extracellular domain. |
| Isotype | IgG2aκ |
| Species Reactivity |
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| Antibody Type | Monoclonal Antibody |
| Entrez Gene Number |
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| Gene Symbol |
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| Purification Method | Protein G Purified |
| UniProt Number |
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| Molecular Weight | ~58 kDa observed. Target band size appears larger than the calculated molecular weights of 53.39 kDa (human) and 53.88 kDa (mouse) due to glycosylation. Uncharacterized band(s) may appear in some lysates. |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Western Blotting in mouse thymus lysate. Western Blotting Analysis: 4.0 µg/mL of this antibody detected DC-STAMP in 10 µg of mouse thymus lysate. |
| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. |
| Packaging Information | |
|---|---|
| Material Size | 100 µL |