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Millipore This Anti-Pro-Bdnf Antibody, Clone 5d4.1 Is Validated For Use In Western Blotting And Immunohistochemistry (Paraffin) For The Detection Of Pro-Bdnf. - Mill (Additional S&H or Hazmat Fees May Apply)
Brain Derived Neurotrophic Factor (BDNF) is a secreted protein that promotes the survival of neuronal populations that are all located either in the central nervous system or directly connected to it. It is a major regulator of synaptic transmission and plasticity at adult synapses in many regions of the CNS. The versatility of BDNF is emphasized by its contribution to a range of adaptive neuronal responses including long-term potentiation (LTP), long-term depression (LTD), certain forms of short-term synaptic plasticity, as well as homeostatic regulation of intrinsic neuronal excitability. It is induced by cortical neurons, and is necessary for survival of striatal neurons in the brain. Expression of this gene is reduced in both Alzheimer’s and Huntington disease patients. BDNF may play a role in the regulation of stress response and in the biology of mood disorders. BDNF exists as monomers or homodimers and binds to TRKB/NTRK2 receptors.
Product Information
Format
Purified
Presentation
Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality Level
MQ100
Applications
Application
This Anti-pro-BDNF Antibody, clone 5D4.1 is validated for use in Western Blotting and Immunohistochemistry (Paraffin) for the detection of pro-BDNF.
Key Applications
Western Blotting
Immunohistochemistry (Paraffin)
Application Notes
Western Blotting Analysis: A 0.5 µg/mL dilution from a representative lot detected 10 µg of recombinant protein Pro-BDNF but not recombinant protein BDNF. Immunohistochemistry Analysis: A 1:2000 dilution from a representative lot detected Pro-BDNF in human cerebral cortex and thalamic tissue.
Biological Information
Immunogen
GST-tagged recombinant protein corresponding to Human pro-BDNF.
Evaluated by Western Blotting in Rat Brain cell lysate.
Western Blotting Analysis: A 0.5 µg/mL diultion of this antibody detected pro-BDNF in 200 µg of Rat Brain cell lysate.
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
