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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H, R | WB, IH(P) | M | Purified | Monoclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | MABN1185 |
| Description | Anti-Tau (4-repeat isoform RD4) Antibody, clone 7D12.1 |
| Alternate Names |
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| Background Information | Microtubule-associated protein tau (UniProt P10636; also known as Neurofibrillary tangle protein, Paired helical filament-tau, PHF-tau) is encoded by the MAPT (also known as MAPTL, MTBT1, TAU) gene (Gene ID 4137) in human. Extracellular plaque deposits composed of amyloid-β and intracellular neurofibrillary tangles (NFTs) composed of truncated and hyperphosphorylated tau are two neuropathological hallmarks of Alzheimers disease (AD). Tau pathology (tauopathy) can cause toxicity when the brain is devoid of amyloid plaques, and tangle pathology correlates better with clinical dementia than amyloid pathology. Abnormal processing of tau by hyperphosphorylation and proteolytic truncation contribute to the toxicity of tau. Tau also undergoes other types of posttranslational modifications, including glycosylation, ubiquitination, glycation, polyamination, nitration, and lysine methylation, which are believed to be important for its non-pathological functions, including polymerization and stabilization of microtubules. Alternative splicings result in multiple Tau isoforms, including five human Tau spliced isoforms with four C-terminal microtubule-binding repeats/domains (RD4) and four spliced isoforms that contain only three microtubule-binding domains (RD3) due to the absence of exon 10-conding sequence. Isoform-specific antibodies are useful tools for analyzing tau isoforms expression and distribution as well as pathological changes in the human brain. |
| Product Information | |
|---|---|
| Format | Purified |
| Presentation | Purified mouse monoclonal IgG1κ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | This Anti-Tau Antibody, clone 7D12.1 is validated for use in Western Blotting, Immunohistochemistry (Paraffin) for the detection of Tau (4-repeat isoform RD4). |
| Key Applications |
|
| Application Notes | Immunohistochemistry Analysis: A 1:50-250 dilution from a representative lot detected Tau 4-repeat isoforms in Alzheimers diseased (AD) human brain, as well as human kidney and non-AD brain tissue sections. |
| Biological Information | |
|---|---|
| Immunogen | KLH-conjugated synthetic peptide (VQIINKKLDLSNVQSKC) corresponding to a junction-flanking sequence coded by adjacent exons 9 & 10 of Tau. The immunizing sequence is identical in human, rat, mouse and bovine. |
| Epitope | Junction-flanking region coded by adjacent exons 9 & 10. |
| Clone | 7D12.1 |
| Concentration | Please refer to lot specific datasheet. |
| Host | Mouse |
| Specificity | Clone 7D12.1 targets an epitope sequence present in Tau spliced isoforms with four C-terminal microtubule-binding repeats/domains (RD4), including human isoforms PNS-tau, Tau-D, Tau-E, Tau-F, and Tau-G, but not Tau spliced isoforms that contain only three microtubule-binding domains (RD3) due to the absence of exon 10-conding sequence (human isoforms Fetal-tau, Tau-A, Tau-B, and Tau-C). |
| Isotype | IgG1κ |
| Species Reactivity |
|
| Species Reactivity Note | Human, Rat. Predicted to react with Bovine, Mouse based on 100% sequence homology. |
| Antibody Type | Monoclonal Antibody |
| Entrez Gene Number |
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| Gene Symbol |
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| Purification Method | Protein G purified. |
| UniProt Number |
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| Molecular Weight | ~45-58 kDa observed. 78,80 kDa (human isoform 1; PNS-tau), 39.88 kDa (human isoform 6; Tau-D), 42.84 kDa (human isoform 7; Tau-E), 45.72 kDa (human isoform 8; Tau-F), 80.81 kDa (human isoform 9; Tau-G) calculated (Met1 removed). |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Western Blotting in rat brain tissue cytosol lysate. Western Blotting Analysis: 0.1 µg/mL of this antibody detected Tau 4-repeat isoforms in 10 µg of rat brain tissue cytosol lysate. |
| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at 2-8°C from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 100 μg |