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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| R, H | WB, IH(P) | M | Purified | Monoclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | MABN1188 |
| Replaces | MAB318 |
| Description | Anti-Tyrosine Hydroxylase Antibody, clone 10F8.2 |
| Alternate Names |
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| Background Information | Tyrosine 3-monooxygenase (EC 1.14.16.2; UniProt P04177; also known as TH, Tyrosine 3-hydroxylase) is encoded by the Th gene (Gene ID 25085) in rat species. Tyrosine hydroxylase plays an important role in the physiology of adrenergic neurons. It is the first and rate-limiting enzyme involved in the biosynthesis of the catecholamines, dopamine and norepinephrine, from tyrosine. TH is, therefore, a useful marker for dopaminergic and noradrenergic neurons. TH enzymatic activity requires ferrous ions as cofactor and is believed to be regulated by phosphorylation. Six human TH isoforms exist as a result of alternative splicing (UniProt P07101-1 through P07101-6). |
| Product Information | |
|---|---|
| Format | Purified |
| Presentation | Purified mouse monoclonal IgG2bλ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | This Anti-Tyrosine Hydroxylase Antibody, clone 10F8.2 is validated for use in Western Blotting, Immunohistochemistry (Paraffin) for the detection of Tyrosine Hydroxylase. |
| Key Applications |
|
| Application Notes | Immunohistochemistry Analysis: A 1:250 dilution from a representative lot detected tyrosine hydroxylase in rat brain tissue sections. |
| Biological Information | |
|---|---|
| Immunogen | KLH-conjugated linear peptide corresponding to a C-terminal region sequence of rat tyrosine hydroxylase. |
| Epitope | C-terminal region. |
| Clone | 10F8.2 |
| Concentration | Please refer to lot specific datasheet. |
| Host | Mouse |
| Specificity | Clone 10F8.2 targets a C-terminal epitope present in rat and mouse TH sequence (UniProt P04177 & P24529), as well as in all six human TH spliced isoforms (UniProt P07101). |
| Isotype | IgG2bλ |
| Species Reactivity |
|
| Species Reactivity Note | Human, Rat. Predicted to react with Mouse based on 100% sequence homology. |
| Antibody Type | Monoclonal Antibody |
| Entrez Gene Number |
|
| Gene Symbol |
|
| Purification Method | Protein G purified. |
| UniProt Number |
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| Molecular Weight | ~50-60 kDa observed. 55.83 kDa (rat), 55.86 kDa (mouse), 58.03/55.48/58.47/55.92/45.21/44.77 kDa (human isoform 1/2/3/4/5/6) calculated (Met1 removed). The multiplet banding pattern observed in rat PC12 lysate most likely represent differentially phosphorylated TH species (Gozal, E., et al. (2005). J. Appl. Physiol. 99(2):642-649). |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Western Blotting in PC12 cell lysate. Western Blotting Analysis: 0.25 µg/mL of this antibody detected tyrosine hydroxylase in 10 µg of PC12 cell lysate. |
| Usage Statement |
|
| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at 2-8°C from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 100 µL |