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Millipore Detect Roundabout Homolog 2 Using This Mouse Monoclonal Antibody, Anti-Robo2 Antibody, Clone 4c6.1 Validated For Use In Western Blotting & Ihc (Paraffin). - Mill (Additional S&H or Hazmat Fees May Apply)
Roundabout homolog 2 (Robo2) is a receptor for SLIT2, and likely SLIT1, which are thought to help guide cellular migration, including axonal navigation of the neural tube and the projection of axons during neuronal development. Vesicoureteral reflux type 2 (VUR2) has been attributed to defects in Robo2.
Product Information
Format
Purified
Control
Mouse neuronal stem cell lysate
Presentation
Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality Level
MQ100
Applications
Application
Detect Roundabout homolog 2 using this mouse monoclonal antibody, Anti-Robo2 Antibody, clone 4C6.1 validated for use in western blotting & IHC (Paraffin).
Key Applications
Western Blotting
Immunohistochemistry (Paraffin)
Application Notes
Immunohistochemistry Analysis: A 1:500 dilution from a representative lot detected Robo2 in rat brain (hypothalamus) and human brain tissue.
Biological Information
Immunogen
GST-tagged recombinant protein corresponding to the cytoplasmic domain of human Robo2.
Epitope
Cytoplasmic domain
Clone
4C6.1
Concentration
Please refer to the Certificate of Analysis for the lot-specific concentration.
Host
Mouse
Specificity
This antibody recognizes the cytoplasmic domain of Robo2
Isotype
IgG1κ
Species Reactivity
Mouse
Human
Rat
Antibody Type
Monoclonal Antibody
Entrez Gene Number
NP_002933
Gene Symbol
ROBO2
KIAA1568
Purification Method
Protein G Purified
UniProt Number
Q9HCK4
Molecular Weight
~ 170 kDa observed. The calculated molecular weight is 150 kDa, however Robo2 has been shown as a ~170 kDa band in western blots due to high glycosylation. (Fricke, C., et al. (2001). Science. 292:(5516):507-510).
Product Usage Statements
Quality Assurance
Evaluated by Western Blotting in mouse neuronal stem cell lysate.
Western Blotting Analysis: 1.0 µg/mL of this antibody detected Robo2 in 200 µg of mouse neuronal stem cell lysate.
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
