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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H, M | IH(P), ICC, WB, ELISA | M | Purified | Monoclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | MABN1792 |
| Description | Anti-Sortilin Antibody, clone F11 |
| Alternate Names |
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| Background Information | Sortilin (UniProt Q99523; also known as 100 kDa NT receptor, Glycoprotein 95, Gp95, Neurotensin receptor 3, NT3, NTR3) is encoded by the SORT1 (also known as LDLCQ6, NT3) gene (Gene ID 6272) in human. Sortlin belongs to the family of sorting receptors that are characterized by the Vps10p domain, which creates a tunnel cavity with its ten-bladed β-propeller for the binding of soluble ligands. These family members also contain a short cytoplasmic tail that harbors recognition motifs for subcellular sorting adaptor proteins. Sortilin is reported to bind PCSK9 in the trans-Golgi network with high-affinity in HepG2 cells. Sort1 knockout mice exhibit low levels of PCSK9 in plasma and its subcellular localization in hepatocytes is also altered. Sortilin is also found to co-localize with amyloid precursor protein (APP) in neurites and it facilitates APP processing toward production of sAPPα, possibly by optimizing APP as substrate for α-secretase cleavage. Sortilin is initially produced with a signal (a.a. 1-33) and a propeptide (a.a. 34-77) sequence, the removal of which yields the mature receptor (a.a. 78-831) with a large luminal/extracellular (a.a. 78-555) portion, a transmembrane region(a.a. 756-778), and a short cytoplasmic tail (a.a.779-831). Like the other family members (MPRs and SorLA), sortilin resides both on the membrane of intracellular vesicles and on the plasma membrane. Altered sortilin expressions due to SORT1 genetic variations affect serum levels of low density lipoprotein cholesterol (LDL-C) and contribute to the LDL-C level quantitative trait locus 6 (LDLCQ6) and susceptibility to myocardial infarction. |
| Product Information | |
|---|---|
| Format | Purified |
| Presentation | Purified mouse monoclonal IgG1κ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Anti-Sortilin Antibody, clone F11 is an antibody against Sortilin Antibody for use in Immunohistochemistry (Paraffin), Immunocytochemistry, Western Blotting and ELISA. |
| Key Applications |
|
| Application Notes | Immunocytochemistry Analysis: A representative lot detected cellular localization of endogenous sortilin in isolated hippocampal neurons from new born (P0) wild-type, but not Sort1−/− C57BL/6J mice (Gustafsen, C., et al. (2013). J Neurosci. 33(1):64-71). Immunocytochemistry Analysis: Representative lots detected cellular localization of exogenously expressed sortilin in transfected HEK293 cells by fluorescent immunocytochemistry (Larsen, J.V., et al. (2014). Biochem J. 457(2):277-288; Gustafsen, C., et al. (2013). J Neurosci. 33(1):64-71; Larsen, J.V., et al. (2010). Mol. Cell. Biol. 30(17):4175-4187; Nielsen, M.S., et al. (2007). Mol. Cell. Biol. 27(19):6842-6851). ELISA Analysis: A representative lot was employed as the detection antibody in sandwich ELISA. A positive correlation was found between PCSK9 and soluble sortilin in human serum samples from healthy individuals (Gustafsen, C., et al. (2014). Cell Metab. 19(2):310-318). Western Blotting Analysis: A representative lot detected different distribution of Sortilin than SorLA among subcellular fractions obtained by gradient centrifugation (Nielsen, M.S., et al. (2007). Mol. Cell. Biol. 27(19):6842-6851). |
| Biological Information | |
|---|---|
| Immunogen | Recombinant protein corresponding to the extracellular domain of human Sortilin. |
| Epitope | Extracellular domain |
| Clone | F11 |
| Concentration | Please refer to lot specific datasheet. |
| Host | Mouse |
| Isotype | IgG1κ |
| Species Reactivity |
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| Antibody Type | Monoclonal Antibody |
| Entrez Gene Number |
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| Gene Symbol |
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| Purification Method | Protein G Purified |
| UniProt Number |
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| Molecular Weight | ~91 kDa calculated |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Immunohistochemistry in human cerebral cortex tissue. Immunohistochemistry Analysis: A 1:50 dilution of this antibody detected Sortilin in human cerebral cortex tissue. |
| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at 2-8°C from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 100 μg |