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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H | IH(P), WB | M | Purified | Monoclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | MABN1846 |
| Description | Anti-Apelin Receptor/APJ, clone 3C3-7 Antibody |
| Alternate Names |
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| Background Information | Apelin receptor (UniProt P35414; also known as Angiotensin receptor-like 1, G-protein coupled receptor APJ, G-protein coupled receptor HG11) is encoded by the APLNR (also known as AGTRL1, APJ) gene (Gene ID 187) in human. Apelin is an angiotensin converting enzyme 2 (ACE2) substrate that functions as an inotropic and cardiovascular protective peptide. Apelin exerts its physiological effects via its receptor APJ, a seven-transmembrane G protein-coupled receptor that shares significant homology with the Ang II type 1 receptor (AT1R). Apelin/APJ signaling plays an important role in a wide range of pathophysiological effects in the cardiovascular system. Apelin levels are reduced in patients with essential hypertension independent of left ventricular systolic and diastolic dysfunction. Treatment with Apelin has been shown to lower systolic blood pressure levels in hypertensive rats via an NO-dependent mechanism. APJ is a 7-transmembrane (a.a. 27-51, 67-91, 101-125, 145-166, 201-221, 245-271, 285-308) GPCR, having a short N-terminal extracellular portion (a.a. 1-26) and a long C-terminal cytomplasmic tail (a.a. 309-380). |
| Product Information | |
|---|---|
| Format | Purified |
| Presentation | Purified mouse monoclonal IgG1κ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | This Anti-Apelin Receptor, clone 3C3-7 Antibody is validated for use in Immunohistochemistry (Paraffin), Western Blotting for the detection of APJ. |
| Key Applications |
|
| Application Notes | Immunohistochemistry Analysis: A 1:250 dilution from a representative lot detected Apelin receptor/APJ in human prostate cancer tissue, as well as normal cerebral cortex and pancreas tissue sections. |
| Biological Information | |
|---|---|
| Immunogen | Recombinant full-length human Apelin receptor/APJ. |
| Clone | 3C3-7 |
| Concentration | Please refer to lot specific datasheet. |
| Host | Mouse |
| Specificity | Clone 3C3-7 detects human Apelin receptor/APJ. |
| Isotype | IgG1κ |
| Species Reactivity |
|
| Species Reactivity Note | Human. |
| Antibody Type | Monoclonal Antibody |
| Entrez Gene Number |
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| Gene Symbol |
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| Purification Method | Protein G purified. |
| UniProt Number |
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| Molecular Weight | ~42 kDa. 42.66 kDa calculated. Uncharacterized band(s) may appear in some lysates. |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Western Blotting in MCF7 cell lysate. Western Blotting Analysis: 1.0 µg/mL of this antibody detected Apelin receptor/APJ in 10 µg of MCF7 cell lysate. |
| Usage Statement |
|
| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at 2-8°C from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 100 μg |