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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H, M, R | WB, IH(P) | M | Purified | Monoclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | MABN313 |
| Description | Anti-DEXRAS1 Antibody, clone 4B11.1 |
| Alternate Names |
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| Background Information | Dexamethasone-induced Ras-related protein 1 (UniProt Q9Y272; also known as Activator of G-protein signaling 1) is encoded by the RASD1 (also known as AGS1, DEXRAS1) gene (Gene ID 51655) in human. Rasd1/Dexras belongs to the monomeric Ras superfamily of GTPases that are commonly referred to as small G-proteins. Rasd1/Dexras1 is a small G protein that functions as an important signal transducer in regulating circadian rhythms, GH secretion, and cell growth. Rasd1/Dexras is rapidly up-regulated by the synthetic glucocorticoid dexamethasone as a result of GR-STAT5b complex-mediated transcription. Rasd1/Dexras1 interacts with neuronal nitric oxide synthase (nNOS or bNOS) via the scaffolding protein CAPON, with nitric oxide serving as a guanine nucleotide exchange factor for Rasd1/Dexras1. Rasd1/Dexras1 is induced by glucocorticoids during adipogenic differentiation. Exogenous Rasd1/Dexras1 expression rescues impaired adipogenesis of Dexras1-deficient mouse embryonic fibroblasts (MEFs). Dexras1 knockout mice display impaired adiposity and are resistant to diet-induced weight gain. Human, moue, and rat Rasd1/Dexras1 protein is initially produced with a 3-amino acid propeptide sequence at the C-terminal end, which was posttranslationally removed to yield the mature protein. |
| Product Information | |
|---|---|
| Format | Purified |
| Presentation | Purified mouse monoclonal IgG2aκ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Anti-DEXRAS1 Antibody, clone 4B11.1 is an antibody against DEXRAS1 for use in Western Blotting, Immunohistochemistry (Paraffin). |
| Key Applications |
|
| Application Notes | Western Blotting Analysis: 1.0 µg/mL from a representative lot detected DEXRAS1 in 10 µg of mouse brain tissue lysate. Immunohistochemistry Analysis: A 1:50-250 dilution from a representative lot detected DEXRAS1 in rat testis, mouse cerebellum, and human kidney tissue sections. |
| Biological Information | |
|---|---|
| Immunogen | GST-tagged recombinant human DEXRAS1 internal fragment. |
| Epitope | Internal. |
| Clone | 4B11.1 |
| Concentration | Please refer to lot specific datasheet. |
| Host | Mouse |
| Specificity | Clone 4B11.1 detects an epitope conserved among human, mouse, and rat RASD1 protein, but missing in human RASD1 spliced isoform 2 reported by UniProt (Q9Y272). |
| Isotype | IgG2aκ |
| Species Reactivity |
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| Antibody Type | Monoclonal Antibody |
| Entrez Gene Number |
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| Gene Symbol |
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| Purification Method | Protein G Purified |
| UniProt Number |
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| Molecular Weight | ~32 kDa oberved. 31.64/31.68/31.71 kDa (human/mouse/rat pro-form) and 31.34/31.38/31.41 kDa (human/mouse/rat mature form) calculated. |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Western Blotting in rat brain tissue lysate. Western Blotting Analysis: 1.0 µg/mL of this antibody detected DEXRAS1 in 10 µg of rat brain tissue lysate. |
| Usage Statement |
|
| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at 2-8°C from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 100 μg |