Special Offers
100% Performance Guaranteed
Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| R, M | IHC | M | Purified | Monoclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | MABN37 |
| Description | Anti-Ataxin-1 Antibody, 11NQ, clone N76/8 |
| Alternate Names |
|
| Background Information | Ataxin-1 is a member of the ATXN1 protein family and contains a single AXH domain. It is a neurodegenerative disorder protein thought to have a role in the metabolism of RNA as it has been shown to localize to the RNA and transcription dependent inclusions within the nucleus. A mutation of Ataxin-1 is the cause of spinocerebellar ataxia type-1 (SCA1), a progressive, neurodegenerative disease that is autosomal dominant and primarily affects the Purjinke cells found in brain stem neuronal populations and the cerebellum. Expression of Ataxin-1 is almost ubiquitous, except in the brain where it is isolated to populations of neurons. |
| Product Information | |
|---|---|
| Format | Purified |
| Control |
|
| Presentation | Purified mouse monoclonal IgG2bκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mm NaCl with 0.05% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Anti-Ataxin-1 Antibody, 11NQ, clone N76/8 detects level of Ataxin-1 & has been published & validated for use in IH. |
| Key Applications |
|
| Application Notes | Western Blot Analysis: A previous lot was used by an independent laboratory in rat brain tissue lysate. (James Trimmer, UC Davis/NIH NeuroMab Facility) Immunohistochemistry Analysis: 1:500 dilution from a previous lot detected Ataxin-1 in rat cerebral cortex tissue. |
| Biological Information | |
|---|---|
| Immunogen | Linear peptide corresponding to mouse Ataxin-1. |
| Clone | N76/8 |
| Concentration | Please refer to the Certificate of Analysis for the lot-specific concentration. |
| Host | Mouse |
| Isotype | IgG2bκ |
| Species Reactivity |
|
| Species Reactivity Note | Demonstrated to react with rat. Predicted to react with mouse based on immunogen design. |
| Antibody Type | Monoclonal Antibody |
| Entrez Gene Number |
|
| Gene Symbol |
|
| Purification Method | Protein g |
| UniProt Number |
|
| UniProt Summary | FUNCTION: May be involved in RNA metabolism. SUBUNIT STRUCTURE: Homooligomer By similarity. Interacts with PQBP1, UBIN and USP7 By similarity. Interacts with ANP32A. Interacts with CIC and ATXN1L. Interacts with ZNF804A (By similarity). SUBCELLULAR LOCATION: Cytoplasm (By similarity). Nucleus. Note: Colocalizes with USP7 in the nucleus (By similarity). TISSUE SPECIFICTY: Widely expressed. In brain, the pattern of distribution is limited to neuron populations. DEVELOPMENTAL STAGE: Transient expression burst in Purkinje cells as the cerebellar cortex becomes functional (postnatal day 14), and in mesenchymal cells of the developing intervertebral disks of the spinal column. DOMAIN: The AXH domain is required for interaction with CIC. PTM: Sumoylation is dependent on nuclear localization and phosphorylation at Ser-751 (By similarity). POLYMORPHISM: The murine poly-Gln region is very limited in comparison to human ATXN1 and is not polymorphic. SEQUENCE SIMILARITIES: Belongs to the ATXN1 family. Contains 1 AXH domain. |
| Molecular Weight | ~ 85 kDa observed |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Immunohistochemistry in rat hippocampus tissue. Immunohistochemistry Analysis: 1:500 dilution of this antibody detected Ataxin-1 in rat hippocampus tissue. |
| Usage Statement |
|
| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at 2-8°C from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 100 µg |