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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H | WB, IHC | M | Culture Supernatant | Monoclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | MABN527 |
| Description | Anti-iNOS/NOS II, clone 13F5.1 Antibody |
| Alternate Names |
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| Background Information | iNOS/NOS II, also known as Nitric oxide synthase inducible, Hepatocyte NOS, HEP-NOS, Inducible NO synthase, Inducible NOS, iNOS, NOS type II, Peptidyl-cysteine S-nitrosylase , and encoded by the gene name NOS2 and NOS2A, produces nitric oxide (NO) which is a messenger molecule with diverse functions throughout the body. Nitric oxide (NO) is an inorganic, gaseous free radical that carries a variety of messages between cells. Vasorelaxation, neurotransmission and cytotoxicity can all be potentiated through cellular response to NO. NO production is mediated by members of the nitric oxide synthase (NOS) family. NOS catalyzes the oxidization of L-arginine to produce L-citrulline and NO. Two constitutive isoforms, brain or neuronal NOS (b or nNOS, type I) & endothelial cell NOS (eNOS, type III), and one inducible isoform (iNOS, type II), have been cloned. Cytokines such as interferon-gamma (IFN), tumor necrosis factor (TNF), interleukin-1 and -2, and lipopolysaccarides (LPS) cause an increase in iNOS mRNA, protein, and activity levels. Protein kinase C-stimulating agents exhibit the same effect on iNOS activity. Human iNOS is regulated by calcium/calmodulin (in contrast with mouse NOS2). iNOS/NOS II is expressed in the liver, retina, bone cells and airway epithelial cells of the lung. |
| Product Information | |
|---|---|
| Format | Culture Supernatant |
| Presentation | Mouse monoclonal IgG1λ in buffer containing 0.05% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | This Anti-iNOS/NOS II antibody is validated for use in WB, IH for the detection of iNOS/NOS II. |
| Key Applications |
|
| Application Notes | Immunohistochemistry Analysis: A 1:50-250 dilution from a representative lot detected iNOS/NOS II in human cerebral cortex, human cerebellum, and human colon tissue. |
| Biological Information | |
|---|---|
| Immunogen | GST-tagged recombinant protein corresponding to human iNOS/NOS II. |
| Clone | 13F5.1 |
| Concentration | Please refer to lot specific datasheet. |
| Host | Mouse |
| Isotype | IgG1λ |
| Species Reactivity |
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| Antibody Type | Monoclonal Antibody |
| Entrez Gene Number |
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| Gene Symbol |
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| Purification Method | Unpurified |
| UniProt Number |
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| Molecular Weight | ~130 kDa observed. Uncharacterized band(s) may appear in some lysates. |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Western Blotting in human small intestine tissue lysate. Western Blotting Analysis: A 1:500 dilution of this antibody detected iNOS/NOS II in 10 µg of human small Intestine tissue lysate. |
| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. |
| Packaging Information | |
|---|---|
| Material Size | 100 µL |