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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H | IHC, DB, ELISA, IP, IF | Camel | Alkaline Phosphatase | Monoclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | MABN687 |
| Description | Anti-β-amyloid fibril-specific, clone B10, AP Antibody |
| Alternate Names |
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| Background Information | Amyloid fibrils are naturally occurring polypeptide scaffolds with considerable importance for human health and disease. A recombinant antibody domain fragment termed B10 specifically recognizes an amyloid-specific and conformationally defined epitope. The specificity and conformational specificity have been established by various methods, including, surface plasmon resonance, immunoblots, and immunohistochemistry. All these methods demonstrate that this antibody domain fragment distinguishes Aβ amyloid fibrils from disaggregated Aβ peptide as well as from specific Aβ oligomers. The antibody domain also possesses functional activity in preventing the formation of mature amyloid fibrils by stabilizing Aβ protofibrils, and recent data suggests that the B10 antibody fragment selectively binds to Alzheimers Aβ(1-40) amyloid fibrils and that fibril recognition depends on positively charged residues within the B10 antigen binding site. Mutation or alternation of specific residues changes B10’s interactions with various fibril configurations, implying that the B10 conformational specificity for amyloid fibrils depends upon specific electrostatic interactions with an acidic moiety, which is common to different amyloid fibrils. |
| Product Information | |
|---|---|
| Format | Alkaline Phosphatase |
| Presentation | Purified Camelid monoclonal IgG in buffer containing 20mM NaH2PO4, 175mM NaCl, pH8.0 without preservatives. Note: This is a Camelid antibody fused to an alkaline phosphatase and does not require a secondary antibody for detection. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Anti-β-amyloid fibril-specific, clone B10, AP | MABN687 is an antibody against β-amyloid fibril-specific for use in Immunohistochemistry, DOT Blot, ELISA, Immunoprecipitation, Immunofluorescence. |
| Key Applications |
|
| Application Notes | This is a Camelid antibody fused to an alkaline phosphatase and does not require a secondary antibody for detection. DOT Blot Analysis: A representative lot detected β-amyloid fibril-specific in synthetic Aβ (1–40) peptide (Habicht, g., et Al. (2007). PNAS. 104(49):19232-19237). DOT Blot Analysis: A representative lot detected β-amyloid fibril-specific in chemically modified fibrils (Haupt, C., et Al. (2011). J. Mole. Biol. 405:341-348). ELISA Analysis: A representative lot detected β-amyloid fibril-specific in N-biotinylated Aβ (1–40) conformers (disaggregated peptide, oligomers, or fibrils) (Morgado, I., et Al. (2012). PNAS. 109(31):12503-12508). Immunohistochemistry Analysis: A representative lot detected β-amyloid fibril-specific in Hippocampal sections from Alzheimer brain tissue (Habicht, g., et Al. (2007). PNAS. 104(49):19232-19237). Immunoprecipitation Analysis: A representative lot detected β-amyloid fibril-specific in native soluble and dispersible fractions from the brain lysates (Upadhaya, A.R., et Al. (2014). BRAIN. 1-17). Immunofluorescence Analysis: A representative lot detected β-amyloid fibril-specific in cell culture-derived amyloid plaques (Habicht, g., et Al. (2007). PNAS. 104(49):19232-19237). |
| Biological Information | |
|---|---|
| Epitope | AB (1-40) amyloid fibrils |
| Clone | B10 |
| Concentration | Please refer to lot specific datasheet. |
| Host | Camel |
| Isotype | IgG |
| Species Reactivity |
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| Antibody Type | Monoclonal Antibody |
| Entrez Gene Number |
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| Gene Symbol |
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| Purification Method | Ni-NTA agarose beads and Mono Q column |
| UniProt Number |
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| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Immunohistochemistry in human Alzheimers brain tissue. Immunohistochemistry Analysis: A 1:50 dilution of this antibody detected β-amyloid fibril-specific in human Alzheimers brain tissue. |
| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. |
| Packaging Information | |
|---|---|
| Material Size | 200 µL |