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Millipore This Anti-Retinal Dehydrogenase 1/Aldh1a1 Antibody, Clone 8d7.1 Is Validated For Use In Western Blotting And Immunohistochemistry (Paraffin) For The Detection Of Retinal Dehydrogenase 1/Aldh1a1. - Mill (Additional S&H or Hazmat Fees May Apply)
Retinal dehydrogenase 1 (EC 1.2.1.36; UniProt P00352; also known as Aldehyde dehydrogenase cytosolic, Aldehyde dehydrogenase family 1 member A1, ALDH-E1, ALHDII, RALDH 1, RalDH1) is encoded by the ALDH1A1 (also known as ALDC, ALDH1, PUMB1) gene (Gene ID 216) in human. ALDH1A1, ALDH1A2, and ALDH1A3 constitute the ALDH1A subfamily of aldehyde dehydrogenases that catalyze the oxidation of all-trans retinal and 9-cis retinal to all-trans retinoic acid and 9-cis retinoic acid, respectively. In addition to its role in detoxifing 4-hydroxy-2-nonenal (4-HNE) in the lens and cornea, ALDH1A1 is also involved in many other biological processes, including stem cell maintenance, UV-radiation resistance, melanogenesis, and spermatogenesis, by catalyzing the conversion of specific aldehydes to their corresponding carboxylic acids.
Product Information
Format
Purified
Presentation
Purified mouse monoclonal IgG1k antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality Level
MQ100
Applications
Application
This Anti-Retinal Dehydrogenase 1/ALDH1A1 Antibody, clone 8D7.1 is validated for use in Western Blotting and Immunohistochemistry (Paraffin) for the detection of Retinal Dehydrogenase 1/ALDH1A1.
Key Applications
Western Blotting
Immunohistochemistry (Paraffin)
Application Notes
Western Blotting Analysis: 1.0 µg/mL from a representative lot detected Retinal Dehydrogenase 1/ALDH1A1 in 10 µg of human cerebellum tissue lysate. Immunohistochemistry Analysis: A 1:250 dilution from a representative lot detected Retinal Dehydrogenase 1/ALDH1A1 in human testis tissue.
Biological Information
Immunogen
GST-tagged recombinant protein corresponding to the N-terminal of human Retinal Dehydrogenase 1/ALDH1A1.
Epitope
N-terminal
Clone
8D7.1
Concentration
Please refer to lot specific datasheet.
Host
Mouse
Isotype
IgG1κ
Species Reactivity
Human
Antibody Type
Monoclonal Antibody
Entrez Gene Number
NP_000680
Gene Symbol
ALDH1A1
ALDC
ALDH1
PUMB1
Purification Method
Protein G Purified
UniProt Number
P00352
Molecular Weight
~55 kDa observed
Product Usage Statements
Quality Assurance
Evaluated by Western Blotting in HepG2 cell lysate.
Western Blotting Analysis: 1.0 µg/mL of this antibody detected Retinal Dehydrogenase 1/ALDH1A1 in 10 µg of HepG2 cell lysate.
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
