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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| R | WB, IHC, RIA, EM, IP, ELISA | M | Purified | Monoclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | MABN845 |
| Description | Anti-Neurophysin 2/NP-AVP Antibody, clone PS 41 |
| Alternate Names |
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| Background Information | The neurophysin 1 and 2 (NPI and NPII) are carriers of peptide hormone oxytocin (OT) and vasopressin (AVP), respectively. Each NP and its associated hormone peptide are synthesized as one precursor protein, OT-NPI (UniProt P01179) encoded by the Oxt (or Ot) gene or AVP-NPII (UniProt P01186) encoded by the Avp gene in rat species. Post-translational cleavages remove the signal peptide and process the precursor into mature OT and NPI (aa 20-28 and aa 32-135, respectively, of OT-NPI) or AVP and NPII (aa 24-32 and aa 36-128, respectively, of AVP-NPII). OT is a potent hormone and neurotransmitter secreted within the pituitary gland. OT exerts its biological activity through its receptor OXTR (also known as OTR). OT is used clinically for labor induction and may exhibit therapeutic potential for treating social anxiety disorders. AVP functions primarily as an anti-diuretic and regulates the concentration of water, glucose, and salts in blood. AVP is secreted from the posterior pituitary, but exerts its biological activity in the kidney via the G protein-coupled receptor AVPR2 (V2R). |
| Product Information | |
|---|---|
| Format | Purified |
| Presentation | Purified mouse monoclonal IgG2bκ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | This Anti-Neurophysin 2/NP-AVP Antibody, clone PS 41 is validated for use in Western Blotting and Immunohistochemistry, Radioimmunoassay, Electron Microscopy, Immunoprecipitation and ELISA for the detection of Neurophysin 2/NP-AVP. |
| Key Applications |
|
| Application Notes | Immunohistochemistry Analysis: A 1:1,000 dilution from a representative lot detected Neurophysin 2/NP-AVP in rat pituitary tissue. Immunohistochemistry Analysis: A representative lot detected Neurophysin 2/NP-AVP immunoreactivity in the hypothalamus of developing rats as early as embryonic day (E16) using coronal vibratome sections. (Witnall, M.H., et al.(1985). J Neurosci. 5(1):98-109). Radioimmunoassay Analysis: Target reactivity of a representative lot was confirmed by RIA using plates coated with purified rat Neurophysin/NP and 125I-labelled secondary antibody (Ben-Barak, Y., et al. (1985). J Neurosci. 5(1):81-97). Electron Microscopy Analysis: A representative lot immunostained granulated neurosecretory vesicles (NSVs) in all axons, Herring bodies, and terminals by EM using rat posterior pituitary LR White sections (Ben-Barak, Y., et al. (1985). J Neurosci. 5(1):81-97). Immunoprecipitation Analysis: A representative lot immunoprecipitated radiolabelled neurophysin 2, but not neurophysin 1 (Ben-Barak, Y., et al. (1985). J Neurosci. 5(1):81-97). ELISA Analysis: A representative lot detected neurophysin 2 immunoreactivity in rat neurophysin preparations by non-sandwich ELISA using either 125I-labelled or HRP-conjugated secondary antibody (Ben-Barak, Y., et al. (1985). J Neurosci. 5(1):81-97). |
| Biological Information | |
|---|---|
| Immunogen | KLH-conjugated acid soluble extract of rat (Sprague-Dawley) intermediate lobes. |
| Epitope | Neurophysin 2 |
| Clone | PS 41 |
| Concentration | Please refer to lot specific datasheet. |
| Host | Mouse |
| Specificity | Clone PS 41 reacts with Vasopressin-neurophysin 2-copeptin precursor, neurophysin 2, but not Arg-vasopressin, copeptin, oxytocin, or neurophysin 1. |
| Isotype | IgG2bκ |
| Species Reactivity |
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| Antibody Type | Monoclonal Antibody |
| Entrez Gene Number |
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| Gene Symbol |
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| Purification Method | Protein G Purified |
| UniProt Number |
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| Molecular Weight | ~15 kDa observed |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Western Blotting in rat pituitary tissue lysate. Western Blotting Analysis: 0.5 µg/mL of this antibody detected Neurophysin 2/NP-AVP in 10 µg of rat pituitary tissue lysate. |
| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at 2-8°C from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 100 μg |