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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| R, H, R | IHC | M | Purified | Monoclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | MABN88 |
| Description | Anti-K+/Cl- Cotransporter (KCC2) Antibody, clone N1/12 |
| Alternate Names |
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| Background Information | KCC2 is a member of the cation-chloride cotransporter gene family. It acts as a K-Cl cotransporter. KCCs normally lower intracellular chloride concentrations below the electrochemical equilibrium potential and depending on the chemical concentration gradients of potassium and chloride, KCC2 can operate as a net efflux or influx pathway. It is proposed to act as the main chloride extruder to promote fast hyperpolarizing postsynaptic inhibition in the brain. KCC2 is expressed at high levels in neurons throughout the nervous system and immunofluorescence shows that the protein is localized at inhibitory synapses of the spinal cord. Studies in mice have shown that KCC2 reduces GABAs inhibitory signaling, resulting in motor defects, epilepsy, and anxiety-like behavior. |
| Product Information | |
|---|---|
| Format | Purified |
| Control |
|
| Presentation | Purified mouse monoclonal IgG2aκ in buffer containing 0.1 M Tris-Glycine (pH 7.4, 150 mm NaCl) with 0.05% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Detect K+/Cl-Cotransporter (KCC2) using this Anti-K+/Cl-Cotransporter (KCC2) Antibody, clone N1/12 validated for use in IH. |
| Key Applications |
|
| Application Notes | Immunohistochemistry Analysis: 1:300 dilution from a previous lot detected KCC2 in rat hypothalamus tissue. |
| Biological Information | |
|---|---|
| Immunogen | Recombinant protein corresponding to rat KCC2. |
| Epitope | Unknown |
| Clone | N1/12 |
| Concentration | Please refer to the Certificate of Analysis for the lot-specific concentration. |
| Host | Mouse |
| Specificity | This antibody recognizes KCC2 |
| Isotype | IgG2aκ |
| Species Reactivity |
|
| Species Reactivity Note | Demonstrated to react with rat. Shown by an independent laboratory to react in human and mouse tissue. (UC Davis/NIH NeuroMab Facility Department of Neurobiology, Physiology and Behavior) |
| Antibody Type | Monoclonal Antibody |
| Entrez Gene Number |
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| Entrez Gene Summary | Neuronal specific potassium-chloride cotransporter isoform; may play a role in response to neuronal injury [RGD]. |
| Gene Symbol |
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| Purification Method | Protein g |
| UniProt Number |
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| UniProt Summary | FUNCTION: Mediates electroneutral potassium-chloride cotransport in mature neurons. Transport occurs under isotonic conditions, but is activated 20-fold by cell swelling. Important for Cl- homeostasis in neurons. Necessary for the ontogenic change in response to gamma-aminobutyric acid (GABA) from depolarization to hyperpolarization during neuronal development. SUBUNIT STRUCTURE: Homomultimer and heteromultimer with other K-Cl cotransporters. Interacts with AP2A1 (By similarity). SUBCELLULAR LOCATION: Membrane; Multi-pass membrane protein. TISSUE SPECIFICTY: Highly expressed in brain. Not detected in other tissues. Highly expressed in pyramidal neurons and in neurons throughout the cortex, hippocampus, the granular layer of the cerebellum and in groups of neurons throughout the brainstem. Barely detectable in dorsal-root ganglions. DEVELOPMENTAL STAGE: Detected in thalamus, but not in hippocampus and neocortex at E20. At birth barely detectable in hippocampus. Expression increases steeply from day 5 to day 9 and then stabilizes at adult levels. SEQUENCE SIMILARITIES: Belongs to the SLC12A transporter family. |
| Molecular Weight | 126 kDa Calculated |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Immunohistochemistry in rat cortex area A-3 tissue. Immunohistochemistry Analysis: 1:300 dilution of this antibody detected KCC2 in rat cortex area A-3 tissue. |
| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at 2-8°C from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 100 µg |