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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| M, H, R | WB, IH(P) | M | Purified | Monoclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | MABS1164 |
| Description | Anti-Nucleobindin-2/Nestafin-1 Antibody, clone 3B3.1 |
| Alternate Names |
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| Background Information | Nucleobindin-2 (UniProt Q9JI85; also known as DNA-binding protein NEFA, Prepronesfatin) is encoded by the Nucb2 (also known as Nefa) gene (Gene ID 59295) in rat species. Nucleobindin-2 is initially translated as a 420-a.a. precursor protein. Cleavage of signal peptide (aa1-24) yields the 396-a.a. mature nucleobindin-2 with very high sequence homology among human, mouse, and rat species (more than 85%). Post-translational processing by prohormone convertases (PC 1/3 and PC 2) cleaves nucleobindin-2 into nesfatin-1, nesfatin-2, and nesfatin-3 (aa1–82, aa85–163, and aa166–396, respectively, numbering based on mature sequence). Nesfatin-1 is a well recognized circulating anorexigenic peptide, whose production increases post-prandially. Nucleobindin-2 contains two calcium-binding EF-hand domains separated by an acidic amino acid-rich region, and a leucine zipper. Nucleobindin-2 is known to be expressed in key hypothalamic nuclei and plays a role in energy homeostasis. Nucleobindin-2 is also expressed in various human peripheral tissues, including the stomach, pancreas, reproductive organs, and adipose tissues, suggesting roles in various metabolic functions. Nucleobindin-2 expression level is reported to be elevated in clear cell renal cell carcinoma (ccRCC), breast cancer, and prostate cancer (PCa) tissues. |
| Product Information | |
|---|---|
| Format | Purified |
| Presentation | Purified mouse monoclonal IgG1κ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | This Anti-Nucleobindin-2 Antibody, clone 3B3.1 is validated for use in Western Blotting, Immunohistochemistry (Paraffin) for the detection of Nestafin-1. |
| Key Applications |
|
| Application Notes | Immunohistochemistry Analysis: A 1:50-250 dilution from a representative lot detected nucleobindin-2/nestafin-1 immunoreactivity in human colon cancer tissue, as well as in normal testis, tonsil, and pancreas tissue sections. |
| Biological Information | |
|---|---|
| Immunogen | GST-tagged recombinant rat Nestafin-1. |
| Epitope | Nesfatin-1 |
| Clone | 3B3.1 |
| Concentration | Please refer to lot specific datasheet. |
| Host | Mouse |
| Specificity | Clone 3B3.1 reacts with pro-nucleobindin-2, mature nucleobindin-2, and nesfatin-1, but not nesfatin-2 or nesfatin-3. |
| Isotype | IgG1κ |
| Species Reactivity |
|
| Species Reactivity Note | Mouse, Human, Rat. |
| Antibody Type | Monoclonal Antibody |
| Entrez Gene Number |
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| Gene Symbol |
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| Purification Method | Protein G purified |
| UniProt Number |
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| Molecular Weight | ~50 kDa observed. 47.33/50.20 kDa (human mature/pro-nucleobindin-2), 47.42/50.30 kDa (mouse mature/pro-nucleobindin-2), 47.25/50.09 kDa (rat mature/pro-nucleobindin-2), 9.552/9.612/9.583 kDa (human/mouse/rat nesfatin-1) calculated. |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Western Blotting in mouse liver tissue lysate. Western Blotting Analysis: 0.5 µg/mL of this antibody detected nucleobindin-2 in 10 µg of mouse liver tissue lysate. |
| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at 2-8°C from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 100 μg |