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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H, M | IHC, IP, IH(P), ELISA, EM | R | Purified | Monoclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | MABS1245 |
| Description | Anti-Thrombomodulin Antibody, clone 273-34A |
| Alternate Names |
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| Background Information | Thrombomodulin (UniProt P15306; also known as CD141, Fetomodulin, SnoRNA MBII-339, TM) is encoded by the Thbd gene (Gene ID 21824) in murine species. Thrombomodulin (TM) is an endothelial cell surface protein that function to suppress the propagation of thrombin (activated blood-coagulation factor II) generation by acting as a thrombin receptor. Formation of the TM-thrombin complex abolishes thrombins procoagulant activity by altering its substrate specificity, leading to proteolytic cleavage of protein C into the natural anticoagulant termed "activated protein C". Together with protein S, activated protein C proteolytically cleaves activated coagulation factors Va and VIIIa, thereby preventing the generation of activated coagulation factors Xa and thrombin. Murine thrombomodulin is initially produced with an N-terminal propeptide (a.a. 1-16) sequence, the removal of which yields the mature protein with a large extracellular region (a.a. 17-517), a transmembrane segment (a.a. 518-541), and a short cytoplamic tail (a.a. 542-577). |
| Product Information | |
|---|---|
| Format | Purified |
| Presentation | Purified rat monoclonal IgG2aκ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | This Anti-Thrombomodulin Antibody, clone 273-34A is validated for use in Immunohistochemistry, Immunoprecipitation, Immunohistochemistry (Paraffin), ELISA, Electron Microscopy for the detection of Thrombomodulin. |
| Key Applications |
|
| Application Notes | Immunohistochemistry Analysis: A 1:50 dilution from a representative lot detected Thrombomodulin in human kidney tissue. Immunoprecipitation Analysis: Representative lots immunoprecipitated the 112 kDa thrombomodulin from normal mouse lung fibroblasts (NMLF), but not Line 1 mouse lung carcinoma cells (Kennel, S.J., et al. (1988). Lab. Invest. 59(5):692-701; Kennel, S.J., et al. (1987). Exp. Mol. Pathol. 47(1):110-124). Immunohistochemistry Analysis: A representative lot detected upregulated thrombomodulin immunoreactivity associated with the neoepidermis during wound healing using formalin-fixed, paraffin-embedded mouse skin sections (Peterson, J.J., et al. (1999). Am. J. Pathol. 155(5): 1569–1575). Immunohistochemistry Analysis: Representative lots detected epithelium thrombomodulin immunoreactivity in paraffin-embedded mouse lung sections (Ford, V.A., et al. (1992). J. Biol. Chem. 267(8):5446-5450; Kennel, S.J., et al. (1987). Exp. Mol. Pathol. 47(1):110-124). Immunohistochemistry Analysis: A representative lot detected thrombomodulin immunoreactivity associated with capillary endothelial cells by fluorescent immunohistochemistry using frozen mouse lung sections (Kennel, S.J., et al. (1987). Exp. Mol. Pathol. 47(1):110-124) ELISA Analysis: Representative lots were used as the capture antibody for the detection of thrombomodulin in mouse tissue samples by sandwich ELISA using clone 411-201B (Cat. No. MABS1273) as the detection antibody, followed by an HRP-conjugated secondary antibody, or by sandwich radio-immunoassay using 125I-labeled clone 411-201B as the detection antibody (Isermann, B., et al. (2001). Development. 128(6):827-838; Conway, E.M., et al. (1999). Blood. 93(10):3442-3450; Weiler-Guettler, H., et al. (1996). Circ. Res. 78(2):180-187; Ford, V.A., et al. (1992). J. Biol. Chem. 267(8):5446-5450). Electron Microscopy Analysis: Representative lots detected thrombomodulin immunoreactivity associated with capillary endothelial cells by EM using frozen mouse lung sections (Kennel, S.J., et al. (1988). Lab. Invest. 59(5):692-701; Kennel, S.J., et al. (1987). Exp. Mol. Pathol. 47(1):110-124). Western Blotting Analysis: A representative lot detected purified thrombomodulin (P112) from normal mouse lung fibroblasts (NMLF) (Kennel, S.J., et al. (1988). Lab. Invest. 59(5):692-701). |
| Biological Information | |
|---|---|
| Immunogen | Normal mouse lung microsomal fraction. |
| Epitope | Extracellular domain |
| Clone | 273-34A |
| Concentration | Please refer to lot specific datasheet. |
| Host | Rat |
| Isotype | IgG2aκ |
| Species Reactivity |
|
| Antibody Type | Monoclonal Antibody |
| Entrez Gene Number |
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| Gene Symbol |
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| Purification Method | Protein G Purified |
| UniProt Number |
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| Molecular Weight | ~ 62 kDa calculated |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Immunohistochemistry in mouse heart tissue. Immunohistochemistry Analysis: A 1:50 dilution of this antibody detected Thrombomodulin in mouse heart tissue. |
| Usage Statement |
|
| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at 2-8°C from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 100 μg |