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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H, M, R | WB, ICC, ELISA, DB | M | Purified | Monoclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | MABS1304 |
| Description | Anti-ATP Synthase subunit β Antibody, clone 11/21-7-A8 |
| Alternate Names |
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| Background Information | ATP synthase subunit beta, mitochondrial (EC 3.6.3.14; UniProt P06576; also known as ATP synthase H+ transporting mitochondrial F1 complex beta polypeptide, beta-F1-ATPase, Epididymis secretory protein Li 271, Mitochondrial ATP synthase beta subunit, Mitochondrial ATP synthetase beta subunit) is encoded by the ATP5B (also known as ATPMB, ATPSB, HEL-S-271) gene (Gene ID 506) in human. Mitochondrial ATP synthase produces ATP from ADP in the presence of a proton gradient generated by electron transport complexes. This ATPase contains two structural domains, F1-containing extramembrane catalytic core, and F0-containing the membrane proton channel that are linked via a central stalk and a peripheral stalk. Subunits alpha and beta form the catalytic code in F1. Tumor development requires the selection of cancer cells with a repressed biogenesis and functional activity of mitochondria. Both beta-F1-ATPase/GAPDH and beta-F1-ATPase/Hsp60 ratios are found to be significantly lower in tumors than the corresponding normal tissues. Studies conducted in human colon cancer cell line HCT116 show the involvement of AMPK (AMP-activated protein kinase) and GCN2 (general control non-derepressible 2; eIF2α kinase) in the onset of colon cancer progression by repressing of beta-F1-ATPase synthesis and promoting the abnormal bioenergetics of mitochondria. |
| Product Information | |
|---|---|
| Format | Purified |
| Presentation | Purified mouse monoclonal IgG1κ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mm NaCl with 0.05% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | This Anti-ATP Synthase subunit β Antibody, clone 11/21-7-A8 is validated for use in Western Blotting, Immunocytochemistry, ELISA and DOT Blot for the detection of ATP Synthase subunit β. |
| Key Applications |
|
| Application Notes | Immunocytochemistry Analysis: A representative lot immunostained mitochondrial tubular network in human breast cancer Hs578T cells (Acebo, P., et Al (2009). Transl Oncol. 2(3):138-145). ELISA Analysis: A representative lot detected His-tagged full-length human ATP Synthase subunit β (beta-F1-ATPase) recombinant protein by direct ELISA (Acebo, P., et Al (2009). Transl Oncol. 2(3):138-145). DOT Blot Analysis: A representative lot detected ATP Synthase subunit β (beta-F1-ATPase) by DOT blot using His-tagged full-length human beta-F1-ATPase recombinant protein or HepG2 lysate (Acebo, P., et Al (2009). Transl Oncol. 2(3):138-145). Western Blotting Analysis: A representative lot detected ATP Synthase subunit β (beta-F1-ATPase) in human hepatoma HepG2, murine hepatoma HEPA 1-6, and normal rat liver epithelial C9 (Clone 9) cells. Western Blotting Analysis: A representative lot detected ATP Synthase subunit β (beta-F1-ATPase) expression in various cancer patients tissues (Acebo, P., et Al (2009). Transl Oncol. 2(3):138-145). Western Blotting Analysis: A representative lot detected ATP Synthase subunit β (beta-F1-ATPase) downregulation in HCT116 human colon cancer cells in response to AMPK pathway activation upon oligomycin or AICAR treatment (Martinez-Reyes, J., et Al. (2012). Biochem J. 444(2):249-259). |
| Biological Information | |
|---|---|
| Immunogen | His-tagged recombinant protein corresponding to human ATP Synthase subunit β. |
| Clone | 11/21-7-A8 |
| Concentration | Please refer to lot specific datasheet. |
| Host | Mouse |
| Isotype | IgG1κ |
| Species Reactivity |
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| Antibody Type | Monoclonal Antibody |
| Entrez Gene Number |
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| Gene Symbol |
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| Purification Method | Protein g Purified |
| UniProt Number |
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| Molecular Weight | ~56 kDa observed |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Western Blotting in HepG2 cell lysate. Western Blotting Analysis: 0.5 µg/mL of this antibody detected ATP Synthase subunit β in 10 µg of HepG2 cell lysate. |
| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at 2-8°C from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 100 μg |