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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H, R | WB | M | Purified | Monoclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | MABS1704 |
| Description | Anti-Potassium Channel Kir6.2 Antibody, clone 10C1.1 |
| Alternate Names |
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| Background Information | ATP-sensitive inward rectifier potassium channel 11 (UniProt Q14654; also known as IKATP, Inward rectifier K(+) channel Kir6.2, Potassium channel, inwardly rectifying subfamily J member 11) is encoded by the KCNJ11 (also known as BIR, HHF2, MODY13, PNDM, TNDM3) gene (Gene ID 3767) in human. Inward rectifier K+ channels (Kirs) modulate cell membrane potential and are classified as K+ transport channels (Kir1.x, Kir4.x, Kir5.x, Kir7.x), classic Kir (Kir2.x), G-protein-gated Kirs (Kir2.x and Kir3.x), and ATP-sensitive Kirs (Kir6.x). Kirs mediate diverse physiological functions of both excitable and nonexcitable epithelial cells, such as cell adhesion-migration, volume regulation, apoptosis, and proliferation. ATP-sensitive potassium (K-ATP) channels are hetero-octamers composed of pore-forming Kir6.x (6.1 or 6.2) subunits and sulfonylurea receptor (SUR1 or SUR2) regulatory subunits. K-ATP channels are regulated by intracellular ATP and ADP concentrations and are highly expressed in cardiomyocytes, where they play crucial roles in ischemic preconditioning (IPC). Potassium channel openers mimic the cardioprotection, while K-ATP channel blocker or Kir6.2-knockout is shown to abolish the effect of IPC in mice. Human Kir6.2 is a 390-amino acid multi-pass membrane (a.a. 69-93, 117-128, 145-166) protein that contains two small extraceullar regions (a.a. 94-116 and 136-144) and a pore-forming domain (a.a. 117-128), having both its N- and C-terminal exposed intracellularly (a.a. 1-68 and a.a. 167-390). |
| Product Information | |
|---|---|
| Format | Purified |
| Presentation | Purified mouse monoclonal IgG1κ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | This Anti-Potassium Channel Kir6.2 Antibody, clone 10C1.1 is validated for use in Western Blotting for the detection of Potassium Channel Kir6.2. |
| Key Applications |
|
| Application Notes | Western Blotting Analysis: A 1:1000 dilution from a representative lot detected potassium channel Kir6.2 in 10 µg of rat pancreas tissue lysate. |
| Biological Information | |
|---|---|
| Immunogen | KLH-conjugated linear peptide corresponding an N-terminal cytoplasmic domain sequence of human potassium channel Kir6.2. |
| Epitope | N-terminal cytoplasmic domain. |
| Clone | 10C1.1 |
| Concentration | Please refer to lot specific datasheet. |
| Host | Mouse |
| Specificity | Clone 10C1.1 targets human Kir6.2 N-terminal cytoplasmic region not present in the alternatively spliced isoform 2. |
| Isotype | IgG1κ |
| Species Reactivity |
|
| Species Reactivity Note | Human, Rat. |
| Antibody Type | Monoclonal Antibody |
| Entrez Gene Number |
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| Gene Symbol |
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| Purification Method | Protein G purified. |
| UniProt Number |
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| Molecular Weight | ~45 kDa observed. 43.54 kDa (human) and 43.61 kDa (rat) calculated. |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Western Blotting in human pancreas tissue lysate. Western Blotting Analysis: A 1:1000 dilution of this antibody detected potassium channel Kir6.2 in 10 µg of human pancreas tissue lysate. |
| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at 2-8°C from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 100 µL |