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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H, M, R | WB, IHC | M | Purified | Monoclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | MABS178 |
| Description | Anti-STK39 Antibody, clone 5H7.1 |
| Alternate Names |
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| Background Information | Serine/threonine-protein kinase 39 (STK39), also known as STE20/SPS-1 related proline alanine-rich kinase (SPAK), is a member of the SPS1 subfamily of STE20 kinases. STK39 is also very highly conserved between species and has been shown to activate the p38 pathways. STK39 also has a caspase cleavage site and these two functions may mean that STK39 plays a role in stress-activated signaling. STK39 expression is up-regulated by androgens in LNCaP human prostate cancer cells and regulated and phosphorylated by WNK1, which controls the stress signaling pathway. It is activated by WNK4 and then has the ability to stimulate the Na-K-2Cl cotransporter, the inactive version does not possess this ability. Recent research has also made a connection between STK39 and regulation of blood pressure. |
| Product Information | |
|---|---|
| Format | Purified |
| Presentation | Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mm NaCl with 0.05% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Anti-STK39 Antibody, clone 5H7.1 is a highly specific mouse monoclonal antibody, that targets STK39 & has been tested in western blotting & IHC. |
| Key Applications |
|
| Application Notes | Western Blotting Analysis: 1.0 µg/mL from a representative lot detected STK39 in 10 µg of mouse and rat brain tissue lysate. Immunohistochemistry Analysis: A 1:500-2,000 dilution from a representative lot detected STK39 in human cerebellum sections and rat hindbrain sections tissue. |
| Biological Information | |
|---|---|
| Immunogen | GST-tagged recombinant protein corresponding to the C-terminus of human STK39. |
| Epitope | C-terminus |
| Clone | 5H7.1 |
| Concentration | Please refer to the Certificate of Analysis for the lot-specific concentration. |
| Host | Mouse |
| Specificity | This antibody recognizes the C-terminus of STK39. |
| Isotype | IgG1κ |
| Species Reactivity |
|
| Antibody Type | Monoclonal Antibody |
| Entrez Gene Number |
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| Gene Symbol |
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| Purification Method | Protein g Purified |
| UniProt Number |
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| Molecular Weight | ~60 kDa observed |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Western Blotting in human brain tissue lysate. Western Blotting Analysis: 1.0 µg/mL of this antibody detected STK39 in 10 µg of human brain tissue lysate. |
| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at 2-8°C from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 100 µg |