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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H, M | WB, ICC, IHC | M | Purified | Monoclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | MABS275 |
| Description | Anti-Gli3 Antibody, clone 5E1 |
| Alternate Names |
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| Background Information | Gli3 can function as both a transcriptional activator and repressor of the sonic hedgehog (Shh) pathway. The full-length Gli3 form (GLI3FL) becomes an activator (GLI3A) after phosphorylation and nuclear translocation. The C-terminally truncated form (GLI3R), acts as a repressor. Proper balance between the Gli3 activator and the repressor specifies limb digit number and identity during development. In concert with TRPS1, Gli3 plays a role in activating chondrocyte proliferation. Gli3 is expressed in several normal adult tissues, including lung, colon, spleen, placenta, testis, and myometrium. Gli3 defects are associated with Greig cephalo-poly-syndactyly syndrome (GCPS), Pallister-Hall syndrome (PHS), polydactyly postaxial type A1 (PAPA1), polydactyly postaxial type B polydactyly (PAPB), and polydactyly preaxial type 4 (POP4). |
| Product Information | |
|---|---|
| Format | Purified |
| Control |
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| Presentation | Purified mouse monoclonal IgMκ in buffer containing PBS with 0.05% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | This Gli3 antibody is validated for use in WB, ICC & IHC for the detection of the Gli3 protein. |
| Key Applications |
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| Application Notes | Immunocytochemistry Analysis: A representative lot detected Gli3 in PK-15, COS-1, and TM4 cells cotransfected with recombinant Gli3 (Hunt, R., et Al. (2007). Hybridoma. 27(4):231-240.). Immunohistochemistry Analysis: A representative lot detected Gli3 in mouse embryo cross-sections (Laht, S., et Al. (2008). Hybridoma. 27(3):167-174.). |
| Biological Information | |
|---|---|
| Immunogen | Recombinant protein corresponding to the putative repressive motif of human GLI3. |
| Clone | 5E1 |
| Host | Mouse |
| Specificity | This antibody recognizes the truncated, repressor form and the full length protein of human Gli3. |
| Isotype | IgMκ |
| Species Reactivity |
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| Antibody Type | Monoclonal Antibody |
| Entrez Gene Number |
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| Gene Symbol |
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| UniProt Number |
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| Molecular Weight | ~75 kDa and ~200 kDa observed. The truncated, repressor form and full length form of this protein has been observed at ~83 kDa and ~190 kDa, respectively (Tanimoto, Y., et Al. (2012). J Biol Chem. 287(25):21429-21438.). Uncharacterized bands may be observed at ~160 kDa and below ~50 kDa in some cell lysates. |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Western Blotting in human thymus tissue lysate. Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected in Gli3 in 10 µg of human thymus tissue lysate. |
| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at 2-8°C from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 100 µL |