The store will not work correctly when cookies are disabled.
We use cookies to make your experience better.To comply with the new e-Privacy directive, we need to ask for your consent to set the cookies. Learn more.
NETA ALERT: Due to inclement weather our offices are closed today, Monday Feb. 23. We will not be shipping orders.
Millipore Anti-Sclerostin Antibody, Clone 7b6.1 | Mabs445 Is An Antibody Against Sclerostin Antibody For Use In Western Blotting And Immunohistochemistry. - Mill (Additional S&H or Hazmat Fees May Apply)
Sclerostin (UniProt Q9BQB4) is encoded by the SOST (also known as CDD, SOST1, VBCH) gene (ORP UNQ2976/PRO7455/PRO7476; Gene ID 50964) in human. Sclerostin is a glycoprotein expressed in osteocytes and functions as a negatively regulator against bone mass by antagonizing low density lipoprotein receptor-related proteins- (LRP5-/LRP6-) mediated WNT/beta-catenin pathway signaling. Sclerostin levels are increased in the post-menopausal women and elderly and in long term immobilized patients. Sclerostin neutralizing antibody is reported to enhance bone formation, mass, and strength in aged male rats. Sclerostin and its inhibitors have potential in prosthetic, regenerative and preventive therapy in dentistry.
Product Information
Format
Culture Supernatant
Presentation
Mouse monoclonal IgG2bκ antibody tissue culture supernatant without preservatives.
Quality Level
MQ100
Applications
Application
Anti-Sclerostin Antibody, clone 7B6.1 | MABS445 is an antibody against Sclerostin Antibody for use in Western Blotting and Immunohistochemistry.
Key Applications
Western Blotting
Immunohistochemistry
Application Notes
Immunohistochemistry Analysis: A 1:250 dilution from a representative lot detected Sclerostin in human kidney, rat liver and human liver tissue.
Biological Information
Immunogen
GST-tagged recombinant protein corresponding to the internal fragment of human Sclerostin.
Epitope
Internal
Clone
7B6.1
Concentration
Please refer to lot specific datasheet.
Host
Mouse
Specificity
Detects Sclerostin isoform 1 (UniProt Q9BQB4-1). Reactivity toward the spliced isoform 2 (UniProt Q9BQB4-2) is likely, but has not been determined.
Isotype
IgG2bκ
Species Reactivity
Human
Rat
Antibody Type
Monoclonal Antibody
Entrez Gene Number
NP_079513
Gene Symbol
SOST
Purification Method
Unpurified
UniProt Number
Q9BQB4
Molecular Weight
24 kDa calculated
Product Usage Statements
Quality Assurance
Evaluated by Western Blotting in GST-tagged Sclerostin recombinant protein.
Western Blotting Analysis: A 1:500 dilution of this antibody detected 0.5 µg of GST-tagged Sclerostin recombinant protein.
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions
Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
