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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H | IP, INHIB | Ch | Purified | Monoclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | MABS456 |
| Replaces | 04-1140 |
| Description | Anti-PTP1B Antibody (Oxidized), clone scFvs 45 |
| Alternate Names |
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| Background Information | PTP1B is the prototypic member of the protein tyrosine phosphatase (PTP) family, which comprises at least 37 proteins. The family is characterized by a catalytic phosphatase domain of approximately 240 amino acids, and includes both transmembrane and cytosolic enzymes. The PTPs have high substrate specificity for phosphotyrosyl proteins. At the primary sequence level, PTPs share little similarity with the protein serine phosphatases, protein threonine phosphatases, or the acid and alkaline phosphatases. PTP1B is a negative regulator of insulin and leptin signal transduction and may be a potential therapeutic target for diabetes and obesity. Insulin also influences the expression of splice variants of PTP1B. |
| Product Information | |
|---|---|
| Format | Purified |
| Presentation | Purified chicken monoclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mm NaCl with 0.05% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Anti-PTP1B Antibody (Oxidized), clone scFvs 45 | MABS456 is an antibody against PTP1B Antibody for use in Immunoprecipitation, Inhibits Activity/Function. |
| Key Applications |
|
| Application Notes | Immunoprecipitation Analysis: This antibody immunoprecipitated PTP1B (Oxidized) recombinant (aa 1-321; WT and CASA mutant) (Haque, A., et Al. (2011). Cell. 147:185-198). Immunoprecipitation Analysis: This antibody immunoprecipitated PTP1B (Oxidized) recombinant (aa 1-321; WT and CASA mutant) (Haque, A., et Al. (2012). Nature America. 7(12):2127-2143). Inhibits Activity/Function Analysis: A representative lot detected PTP1B (Oxidized) in recombinant PTP1B (aa 1-321; WT and CASA mutant) and scFv45) (Haque, A., et Al. (2011). Cell. 147:185-198). Inhibits Activity/Function Analysis: A representative lot detected PTP1B (Oxidized) in recombinant PTP1B (aa 1-321; WT and CASA mutant)and scFv45) (Haque, A., et Al. (2012). Nature America. 7(12):2127-2143). |
| Biological Information | |
|---|---|
| Immunogen | Recombinant protein corresponding to the PTP1B-OX of human PTP1B (Oxidized). |
| Epitope | PTP1B-OX |
| Clone | scFvs 45 |
| Concentration | Please refer to lot specific datasheet. |
| Host | Chicken |
| Specificity | This antibody only reacts with the oxidized form. |
| Species Reactivity |
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| Antibody Type | Monoclonal Antibody |
| Entrez Gene Number |
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| Gene Symbol |
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| Purification Method | Ni-NTA agarose |
| UniProt Number |
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| Molecular Weight | ~37 kDa observed. The calculated molecular weight is 50kDa however PTP1B has been observed at ~37 kDa since it is a truncated form (aa 1-321). Uncharacterized band(s) may appear in some lysates. |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Immunoprecipitation in recombinant PTP1B (aa 1-321; WT and CASA mutant) and scFv45 cell lysate. Immunoprecipitation Analysis: 278 ng of this antibody detected PTP1B (Oxidized) in recombinant PTP1B (aa 1-321; WT and CASA mutant) and scFv45 cell lysate. |
| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at 2-8°C from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 100 µg |