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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H | WB, IP | M | Purified | Monoclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | MABS61 |
| Description | Anti-PARG Antibody, clone D8B10 |
| Alternate Names |
|
| Background Information | Poly(ADP-ribose) glycohydrolase (PARG) is an enzyme possessing both endo- and exoglycosidase activity against poly (ADP-ribose) (PARP), rapidly degrading PARP to release large quantities of free ADP-ribose. PARG is involved in several cellular processes including; apoptosis, DNA repair, cell cycle progression, cell survival and cellular differentiation. During apoptosis, PARG is cleaved by caspase-3 suggesting that PARG activity is regulated during this process. Although encoded by one gene, PARG is present in different cellular localizations as different isoforms: Isoform 1 (111 kDa) is present in the nucleus, Isoform 2 (102 kDA) is in the cytoplasm and Isoform 3 (99 kDa) is mitochondrial. Studies have indicated a critical role for PARG isoform 1 in the quality of sperm chromatin, and subsequent embryonic survival. |
| Product Information | |
|---|---|
| Format | Purified |
| Control |
|
| Presentation | Purified mouse monoclonal IgG2aκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mm NaCl with 0.05% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Anti-PARG Antibody, clone D8B10 is an antibody against PARG for use in WB & IP. |
| Key Applications |
|
| Biological Information | |
|---|---|
| Immunogen | Histidine-tagged recombinant protein corresponding to human PARG. |
| Epitope | Unknown |
| Clone | D8B10 |
| Concentration | Please refer to the Certificate of Analysis for the lot-specific concentration. |
| Host | Mouse |
| Isotype | IgG2aκ |
| Species Reactivity |
|
| Antibody Type | Monoclonal Antibody |
| Entrez Gene Number |
|
| Entrez Gene Summary | Poly(ADP-ribose) glycohydrolase (PARG) is the major enzyme responsible for the catabolism of poly(ADP-ribose), a reversible covalent-modifier of chromosomal proteins. The protein is found in many tissues and may be subject to proteolysis generating smaller, active products. [provided by RefSeq]. |
| Gene Symbol |
|
| Purification Method | Protein g |
| UniProt Number |
|
| UniProt Summary | FUNCTION: Poly(ADP-ribose) synthesized after DNA damage is only present transiently and is rapidly degraded by poly(ADP-ribose) glycohydrolase. Poly(ADP-ribose) metabolism may be required for maintenance of the normal function of neuronal cells. CATALYTIC ACTIVITY: Hydrolyzes poly(ADP-ribose) at glycosidic (1-2) linkage of ribose-ribose bond to produce free ADP-ribose. SUBCELLULAR LOCATION: Isoform 1: Nucleus. Isoform 2: Cytoplasm. Isoform 3: Cytoplasm. TISSUE SPECIFICTY: Ubiquitously expressed. SEQUENCE SIMILARITIES: Belongs to the poly(ADP-ribose) glycohydrolase family. |
| Molecular Weight | ~ 102 kDa observed. Other isoforms may be observed in some lysates at 111 and 99 kDa. |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Western Blot in Jurkat cell lysate. Western Blot Analysis: 0.5 µg/mL of this antibody detected PARG on 10 µg of Jurkat cell lysate. |
| Usage Statement |
|
| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at 2-8°C from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 100 µg |