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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| R | WB | M | Purified | Monoclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | MABS826 |
| Description | Anti-Rsk1 Antibody, clone 3G5.1 |
| Alternate Names |
|
| Background Information | Ribosomal protein S6 kinase alpha-1 (EC 2.7.11.1; UniProt Q15418; also known as 90 kDa ribosomal protein S6 kinase 1, MAP kinase-activated protein kinase 1a, MAPK-activated protein kinase 1a, MAPKAP kinase 1a, MAPKAPK-1a, p90-RSK 1, p90RSK1, p90S6K, Ribosomal S6 kinase 1, RSK-1, S6K-alpha-1) is encoded by the RPS6KA1 (also known as MAPKAPK1A, RSK1) gene (Gene ID 6195) in human. Ribosomal s6 kinases (RSKs) are serine/threonine kinases that play a role in mediating Mek1/2-Erk1/2 mitogen-activated protein (MAP) kinase pathway signaling events. There exist two types of RSKs, p70rsk (a.k.a. S6 Kinase or S6-H1 kinase) and p90rsk (a.k.a. MAPK-activated protein kinase). Six p90rsk-coding genes have been identified in human, RPS6KA1 (MAPKAPK1A, RSK1), RPS6KA2 (MAPKAPK1C, RSK3), RPS6KA3 (MAPKAPK1B, RSK2), RPS6KA4 (MSK2), RPS6KA5 (MSK1), and RPS6KA6 (RSK4). p90RSK1 is activated by sequential phosphorylation events, where it is first phosphorylated by Erk1/2 on the C-terminal kinase domain (CTKD) Thr573, leading to subsequent Ser380 phosphorylation in the N-terminal AGC kinase-type domain. Alternatively, Ser380 can be phosphorylated directly by MK2/3 independent of CTKD Thr573 phosphorylation. |
| Product Information | |
|---|---|
| Format | Purified |
| Presentation | Purified mouse monoclonal IgG1κ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Anti-Rsk1 Antibody, clone 3G5.1 is an antibody against Rsk1 for use in Western Blotting. |
| Key Applications |
|
| Biological Information | |
|---|---|
| Immunogen | KLH-conjugated linear peptide corresponding to a sequence from the linker region between the two human Rsk1 kinase domains. |
| Epitope | Linker region between two kinase domains. |
| Clone | 3G5.1 |
| Concentration | Please refer to lot specific datasheet. |
| Host | Mouse |
| Specificity | Clone 3G5.1 target epitope sequence is present in all four human Rsk1 spliced isoforms reported by UniProt (Q15418). |
| Isotype | IgG1κ |
| Species Reactivity |
|
| Species Reactivity Note | Rat. Predicted to react with Human, Chicken, Bovine, Mouse, Porcine, Xenopus based on 100% sequence homology. |
| Antibody Type | Monoclonal Antibody |
| Entrez Gene Number |
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| Gene Symbol |
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| Purification Method | Protein G purified. |
| UniProt Number |
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| Molecular Weight | ~85 kDa observed. 82.88 kDa (rat), 86.47 kDa (mouse), 82.72 kDa (human isoform 1), 83.93 kDa (human isoform 2), 72.70 kDa (human isoform 3), and 81.15 kDa (human isoform 4) calculated. Uncharacterized band(s) may appear in some lysates. |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Western Blotting in PC12 cell lysate. Western Blotting Analysis: 1.0 µg/mL of this antibody detected Rsk1 in 10 µg of PC12 rat pheochromocytoma cell lysate. |
| Usage Statement |
|
| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at 2-8°C from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 100 μg |