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Millipore Anti-Tom20/Tomm20, Clone 2f8.1 Detects Levels Of Tom20/Tomm20 Proteins & Has Been Published & Validated For Use In Wb & Icc. - Mill (Additional S&H or Hazmat Fees May Apply)
Mitochondrial import receptor subunit TOM20 homolog (TOM20 or TOMM20), also known as mitochondrial 20 kDa outer membrane protein or outer mitochondrial membrane receptor Tom20, is a central component of the pre-protein translocase complex of the outer mitochondrial membrane (TOM complex) which is responsible for the recognition and translocation of cytosolically synthesized mitochondrial pre-proteins. The TOM complex consists of at least 7 different proteins (TOMM5, TOMM6, TOMM7, TOMM20, TOMM22, TOMM40 and TOMM70). TOM20 interacts with TOM22 and facilitates the movement of pre-proteins into the TOM40 translocation pore. Also interacts with APEX1.
Product Information
Format
Purified
Control
HeLa cell lysate
Presentation
Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mm NaCl with 0.05% sodium azide.
Quality Level
MQ100
Applications
Application
Anti-Tom20/Tomm20, clone 2F8.1 detects levels of Tom20/Tomm20 proteins & has been published & validated for use in WB & ICC.
Key Applications
Western Blotting
Immunocytochemistry
Application Notes
Immunocytochemistry Analysis: A 1:500 dilution from a representative lot detected Tom20/Tomm20 in A431, NIH/3T3, and HeLa cells.
Biological Information
Immunogen
GST-tagged recombinant protein corresponding to human Tom20/Tomm20.
Clone
2F8.1
Concentration
Please refer to the Certificate of Analysis for the lot-specific concentration.
Host
Mouse
Isotype
IgG1κ
Species Reactivity
Human
Mouse
Antibody Type
Monoclonal Antibody
Entrez Gene Number
NP_055580
Gene Symbol
TOMM20
KIAA0016
Purification Method
Protein g Purified
UniProt Number
Q15388
Molecular Weight
~ 16 kDa observed
Product Usage Statements
Quality Assurance
Evaluated by Western Blotting in HeLa cell lysate.
Western Blotting Analysis: 0.2 µg/mL of this antibody detected Tom20/Tomm20 in 200 µg of HeLa cell lysate.
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
