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Millipore Detect Integrin Alpha5 (Preservative Free) Using This Anti-Integrin Alpha5 (Preservative Free) Antibody, Clone Snaka51 Validated For Use In Western Blotting, Activity Assay, Icc. - Mill (Additional S&H or Hazmat Fees May Apply)
Integrins are a family of dimeric, transmembrane proteins that mediate cell-cell and extracellular matrix adhesion. Signals transduced by integrins play a role in many biological processes, including cell growth, differentiation, migration and apoptosis. The integrin family is composed of at least 15 alpha and 8 beta subunits that may form over twenty different alpha-beta non-covalently bound dimeric combinations on the cell surface. The alpha subunits all have some homology to each other, as do the beta subunits. Both of the subunits contribute to the binding of the ligand. Integrin alpha subunits contain seven weak sequence repeats in the N-terminal region, which may be important in ligand binding, and have been predicted to fold cooperatively into a single beta-propeller domain with seven beta-sheets. The α5/β1 integrin, also termed VLA5 or CD49e, is identical to the fibronectin receptor and mediates the adhesion of cells to fibronectin.
Product Information
Format
Protein G Purified
Control
HeLa cells
Presentation
Purified mouse monoclonal IgG2aκ in buffer containing PBS without preservatives.
Quality Level
MQ100
Applications
Application
Detect Integrin alpha5 (Preservative Free) using this Anti-Integrin alpha5 (Preservative Free) Antibody, clone SNAKA51 validated for use in Western Blotting, Activity Assay, ICC.
Key Applications
Western Blotting
Activity Assay
Immunocytochemistry
Application Notes
Activity Assay: A representative lot of this antibody converts alpha5 beta1 integrin into a ligand competant form and promotes fibronectin binding (Clark, K., et al. (2005). J Cell Sci. 118(Pt 2):291-300.). Immunocytochemistry Analysis: A representative lot of this antibody detected Integin alpha 5 in Primary human foreskin fibroblasts (hFF) (Clark, K., et al. (2005). J Cell Sci. 118(Pt 2):291-300.; Valdembri, D., et al. (2009). PLoS Biol. 7(1):e25.).
Biological Information
Immunogen
Whole cells corresponding to human Integrin alpha5.
Clone
SNAKA51
Concentration
Please refer to the Certificate of Analysis for the lot-specific concentration.
Host
Mouse
Isotype
IgG2α
Species Reactivity
Human
Antibody Type
Monoclonal Antibody
Entrez Gene Number
NP_001138472
Gene Symbol
ITGAV; MSK8; VNRA
Purification Method
Protein G Purified
UniProt Number
P08648
Molecular Weight
116 kDa calculated
Product Usage Statements
Quality Assurance
Evaluated by Flow Cytometry in HeLa cell treated with fibronectin. Flow Cytometry Analysis: 1 µg of this antibody detected Integrin alpha5 in 0.5X10^6 HeLa cells.
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
