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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H | WB, IP | M | Purified | Monoclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | MABT29 |
| Description | Anti-Ninein Antibody, clone 79-160-7 |
| Alternate Names |
|
| Background Information | Ninein is a coiled-coil, centrosomal protein that is a necessary component in microtubule minus-end anchorage and positioning, and may have a role as a maturation factor for centrosomes. It may also be involved in microtubule nucleation. More recent studies have identified ninein as a crucial component in the formation of blood vessels. Expression of ninein is ubiquitous, but significantly higher levels have been observed in skeletal muscle and heart tissue. There have been 8 isoforms characterized due to alternative splicing. |
| Product Information | |
|---|---|
| Format | Purified |
| Control |
|
| Presentation | Purified mouse monoclonal IgG2aκ in buffer containing 0.1 M Tris-Glycine, pH 7.4, 150 mm NaCl with 0.05% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Anti-Ninein Antibody, clone 79-160-7 is an antibody against Ninein for use in WB & IP. |
| Key Applications |
|
| Application Notes | Immunocytochemistry Analysis: 1:500 dilution of the antibody has been shown to detect Ninein in HeLa and A431 cells. Immunoprecipitation Analysis: The antibody has been shown to immunoprecipitate Ninein in L929 cells. (Delgehyr, 2005). |
| Biological Information | |
|---|---|
| Immunogen | Recombinant protein corresponding to human Ninein. |
| Clone | 79-160-7 |
| Concentration | Please refer to the Certificate of Analysis for the lot-specific concentration. |
| Host | Mouse |
| Isotype | IgG2aκ |
| Species Reactivity |
|
| Antibody Type | Monoclonal Antibody |
| Entrez Gene Number |
|
| Entrez Gene Summary | This gene encodes one of the proteins important for centrosomal function. This protein is important for positioning and anchoring the microtubules minus-ends in epithelial cells. Localization of this protein to the centrosome requires three leucine zippers in the central coiled-coil domain. Multiple alternatively spliced transcript variants that encode different isoforms have been reported. [provided by RefSeq]. |
| Gene Symbol |
|
| Purification Method | Protein g Purified |
| UniProt Number |
|
| UniProt Summary | FUNCTION: Centrosomal protein required in the positioning and anchorage of the microtubule minus-end in epithelial cells. May also act as a centrosome maturation factor. May play a role in microtubule nucleation. Overexpression does not perturb nucleation or elongation of microtubules but suppresses release of microtubules. SUBUNIT STRUCTURE: Homooligomer. Interacts with GSK3B (GSK3-beta) via its C-terminus domain. Interacts with C14ORF166, such interaction may prevent its phosphorylation by GSK3-beta. SUBCELLULAR LOCATION: Cytoplasm › cytoskeleton › centrosome. Note: Component of the core centrosome. Arranged in a tubular conformation with an open and a closed end within the centrosome. In the mother centrosome, it localizes at both ends of the centrosome tube, including the site of centrosome duplication, while in the daughter centrosome it is present only at the closed end. Requires PCM1 for centrosome localization. TISSUE SPECIFICITY: Ubiquitous. Highly expressed in heart and skeletal muscle. Isoform 1 is more expressed than isoform 5. DEVELOPMENTAL STAGE: In interphase cells, it is localized in the centrosome. Decreases in metaphase and anaphase and reappears in telophase. DOMAIN: The coiled coil region from Asn-1611 to Pro-1693 is necessary and sufficient for the targeting to centrosome. PTM: Phosphorylated by Aurora A (AIK) and PKA kinases but not CK2 or Aurora B (AIM). Phosphorylated upon DNA damage, probably by ATM or ATR. MISCELLANEOUS: Antibodies against NIN are present in sera from patients with autoimmune diseases that developed autoantibodies against centrosomal proteins. SEQUENCE SIMILARITIES: Contains 5 EF-hand domains. SEQUENCE CAUTION: The sequence AAH65521.1 differs from that shown. Reason: Miscellaneous discrepancy. Contaminating sequence. Potential poly-A sequence. The sequence BAB13391.2 differs from that shown. Reason: Erroneous initiation. The sequence BAB15640.1 differs from that shown. Reason: Erroneous initiation. |
| Molecular Weight | ~ 250 kDa observed MW. An unidentified nonspecific band appears at ~ 50 kDa. |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Western Blot in Hek293 cell lysate. Western Blot Analysis: 1 µg/mL of the antibody detected Ninein in 10 µg of Hek293 cell lysate. |
| Usage Statement |
|
| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at 2-8°C from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 100 µg |