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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H | WB, ICC, IH(P) | M | Purified | Monoclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | MABT96 |
| Description | Anti-Anillin Antibody, clone 5f3.1 |
| Alternate Names |
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| Background Information | Anillin is a highly conserved multidomain protein that crosslinks multiple structural and regulatory elements including, but not limited to, F-actin, active myosin II, septins, and importin. It positively regulates normal cytokinesis; however, its molecular function within this context remains unclear. Failed cytokinesis results in binucleation, which can lead to genomic instability. In human cultured cells, anillin stabilizes TCA-fixable species of RhoA at the equatorial cortex, but its exact mode of action and subsequent physiological effects have yet to be determined. In some cancers, high levels of septins are shown to out-compete importin binding, thereby promoting the retention of Anillin in the cytoplasm. |
| Product Information | |
|---|---|
| Format | Purified |
| Control |
|
| Presentation | Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Anti-Anillin, clone 5F3.1 is an antibody targeting the Anillin protein, validated for use in WB & ICC. |
| Key Applications |
|
| Application Notes | Immunocytochemistry Analysis: 1 µg/mL of this antibody detected Anillin in the contractile rings of dividing HeLa cells. Immunohistochemistry (Paraffin) Analysis (IHC(P)): A 1:100 dilution of this antibody detected Anillin in human testis tissue sections. |
| Biological Information | |
|---|---|
| Immunogen | GST-tagged recombinant protein corresponding to human Anillin. |
| Clone | 5F3.1 |
| Concentration | Please refer to the Certificate of Analysis for the lot-specific concentration. |
| Host | Mouse |
| Isotype | IgG1κ |
| Species Reactivity |
|
| Antibody Type | Monoclonal Antibody |
| Entrez Gene Number |
|
| Gene Symbol |
|
| Purification Method | Protein G Purified |
| UniProt Number |
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| Molecular Weight | ~160 kDa observed. The calculated molecular weight of this protein is 124 kDa, but can be observed at ~160 kDa due to high glycosylation. Uniprot describes an isoform produced by alternative splicing at ~120 kDa. |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Western Blot in mimosine and nocodozole treated and untreated HeLa cell lysate. Western Blot Analysis: 1.0 µg/mL of this antibody detected Anillin in 10 µg of mimosine and nocodozole treated and untreated HeLa cell lysate. Evaluated by Immunohistochemistry (Paraffin) in human testis tissue sections. Immunohistochemistry (Paraffin) Analysis (IHC(P)): A 1:100 dilution of this antibody detected Anillin in human testis tissue sections. |
| Usage Statement |
|
| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at 2-8°C from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 100 µg |