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Millipore Detect S•Tag Fusion Protein Using This Anti-S•Tag Fusion Protein Antibody Validated For Use In Western Blotting, Icc, Immunoprecipitation. - Mill (Additional S&H or Hazmat Fees May Apply)
The S•Tag fusion protein is one of many polypeptide tags used to facilitate the identification and purification of many targets of interest. The gene encoding the S•Tag peptide can be added into a vector with another gene that encodes a target of interest. Such vectors, which are available through the Novagen® product line, are subsequently transfected into various mammalian, bacterial, or insect cell cultures. Because the target of interest and the S•Tag peptide are expressed as one protein, identification of the target is facilitated with the use of antibodies that are specific for the respective fusion tag. Optional purification of the target may be performed using chromatography techniques that allow for the binding and removal of the respective fusion tag, if desired.
Product Information
Format
Purified
Control
Posi-Tag Epitope Tag Control
Presentation
Purified mouse monoclonal IgG1κ cultured supernatant in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality Level
MQ100
Applications
Application
Detect S•Tag fusion protein using this Anti-S•Tag fusion protein Antibody validated for use in Western Blotting, ICC, Immunoprecipitation.
Key Applications
Western Blotting
Immunocytochemistry
Immunoprecipitation
Application Notes
Immunocytochemistry Analysis: A representative lot detected S•Tag fusion proteins in transfected COS-7 cells.
Immunoprecipitation Analysis: A representative lot immunoprecipitate S•Tag fusion proteins in IP.
Biological Information
Immunogen
Linear peptide corresponding to S•Tag fusion proteins.
Clone
26A4.1.2
Concentration
Please refer to the Certificate of Analysis for the lot-specific concentration.
Host
Mouse
Isotype
IgG1κ
Species Reactivity
All
Antibody Type
Monoclonal Antibody
Purification Method
Protein G Purified
Molecular Weight
~45 kDa observed. The molecular weight of S•Tag fusion protein is not 45 kDa. The Posi-Tag Epitope Tag Control, which contains an S•Tag fusion protein, is observed at ~45 kDa.
Product Usage Statements
Quality Assurance
Evaluated by Western Blot in Posi-Tag Epitope Tag Control.
Western Blot Analysis: 0.05 µg/mL of this antibody detected S•Tag fusion proteins in 5 µL of Posi-Tag Epitope Tag Control.
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
