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Key Specifications Table
Key Applications
Cult
Description
Catalogue Number
PMEF-NL-P1
Trade Name
Embryomax
Specialty Media
Description
EmbryoMax® Primary Mouse Embryonic Fibroblasts
Overview
PMEF-NL-P1 are low passage neomycin resistant Primary Mouse Embryo Fibroblasts (PMEFs) isolated from FVB mouse embryos at day 13. The cells have NOT been mitotically arrested. PMEFs are the traditional feeder cells used to support the growth of undifferentiated mouse and human pluripotent stem cell cultures. Additionally, low passage primary cultures of fibroblasts have a high proliferative capacity and can be used for reprogramming. It is known that cellular reprogramming efficiency declines with each passage, presumably due to lowered proliferative capacity of PMEFs at later passages. These untreated PMEF cells are high quality, low passage (P1) mouse fibroblasts that have been successfully reprogrammed into iPS cells with high efficiency. They can be used to study the mechanisms of reprogramming or be further expanded and treated with γ-irradiation or mitomycin-C to make growth arrested feeder cells. See Murine Embryonic Stem Cell Culture Manual for complete culture and media for PMEF cells.
Alternate Names
EmbryoMax® Primary Mouse Embryonic Fibroblasts
Background Information
The EmbryoMax range of PMEF cells provides researchers with a convenient solution for ES cell culture by eliminating the need for time consuming feeder cell isolation and preparation. Many embryonic stem cell culture protocols necessitate the use of primary mouse embryo fibroblast (PMEF) cells. In these protocols, ES cells are typically cultured on a monolayer of PMEF feeder cells. Feeder cells perform two important roles in stem cell culture: they secrete several important growth factors into the medium, which help maintain pluripotency, and they provide a cellular matrix for ES cells to grow.
Product Information
Presentation
Provided frozen in EMD Millipore Freezing Medium (S-002-D) containing DMSO.
Quality Level
MQ100
Applications
Application
PMEF, Neo Resistant, Strain FVB, Not Treated, Passage 1
Key Applications
Stem Cell Culture
Biological Information
Stem Cell Type
Mouse Embryonic Stem Cells
PMEFs
Induced Pluripotent Stem Cells
Cell Line Type
Primary Mouse Embryo Fibroblasts
Product Usage Statements
Usage Statement
This product contains genetically modified organisms (GMO). Within the EU GMOs are regulated by Directives 2001/18/EC and 2009/41/EC of the European Parliament and of the Council and their national implementation in the member States respectively. This legislation obliges MilliporeSigma to request certain information about you and the establishment where the GMOs are being handled. Click here for Enduser Declaration (EUD) Form.
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions
After receipt, vials should be stored at -80°C. If storing for longer than 6 months, store in the vapor phase of liquid nitrogen (make sure the cap is tight) for up to 2 years.
